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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Hydroalcoholic extract obtained from
Eugenia punicifolia
leaves and its effect in improving injury induced
by gastric ischemia- reperfusion in male and female rats
Périco L L, Rodrigues V P, Santos R C, Ohara R, Vilegas W, Rocha L R M, Santos C
and
Hiruma-Lima C A
University of São Francisco, Brazil
Introduction:
Eugenia punicifolia
(Kunth) DC. (Myrtaceae),
popularly known as “murta”, is a shrub largely distributed
in the Amazon region and Savanna biome. The leaves of this
medicinal plant are popularly used as a natural therapeutic
agent to treat inflammation, wounds and infections.
Aim:
The aim of this study was to evaluate the gastric healing
effect against ulcer induced by ischemia and reperfusion
(I/R).
Material & Methods:
The gastric ulcers were induced by
I/R in male and female (intact and ovariectomized) Wistar
rats, according to the method described by Ueda
et al
.
Hydroalcoholic extract from
Eugenia punicifolia
leaves
(HEEP - 125 mg/kg – lower effective dose of previous
assays, dose-response curve), lansoprazole (30 mg/kg) or
vehicle (saline – 0.9%; 10 mL/kg) were administered during
6 days to determine the healing effects of the subacute
treatment. After treatments, the rats were killed and the
stomach removed for analysis of lesions areas (mm
2
) and
biochemical parameters such as: superoxide dismutase
(SOD - antioxidant), myeloperoxidase (MPO - inflammation
marker), malondialdehyde (MDA - lipid peroxidationmarker),
catalase (CAT - antioxidant) and reduced glutathione (GSH -
antioxidant). The results are expressed as mean ± standard
error of the mean and statistical significance was determined
by ANOVA followed by Dunnett’s test (
p
<0.05). Animal
Research Ethical Committee n. 675.
Results & Discussion:
The results show that the treatment
with lansoprazole and HEEP during 6 consecutive days
significantly healed the gastric ulcers decreasing the lesion
area (males [63.43% and 73.68%]; intact females [68.80%
and 52.83%]; ovariectomized females [50.39% and 43.13%];
respectively)when comparedwith their control group treated
with vehicle. There are no significant changes between
healing area of ovariectomized females and males rats
treated with HEEP for 6 days (
p
>0.05). But when compared
intact females with males, our results showed that the latter
presents decrease in the lesion area after the treatment
with the HEEP (
p
<0.01). Our results indicate that HEEP
administered for 6 days presents curative effects against the
I/R induced lesions increasing GSH levels (
p
<0.0001) in intact
females. The biochemical parameters evaluated in this study
are not related to the healing of the gastric mucosa of males
and ovariectomized females.
Conclusion:
Treatment with HEEP administered during
6 consecutive days in male and female rats (intact and
ovariectomized) after gastric injury induced by I/R, could
heal the mucosa with a significant increase in GSH levels,
acting as antioxidant.
Speaker Biography
Périco L L possess a Bachelor’s degree in Pharmacy from the Faculdades
Adamantinenses Integradas (2010), a Master’s degree in Biological Sciences
(Pharmacology) from the Institute of Biosciences of Botucatu at the São Paulo State
University (UNESP) (2014). She is currently a Doctoral student in Pharmacology and
Biotechnology at Institute of Biosciences of Botucatu (UNESP), where she works on the
following topics: Pharmacology of Natural Products, with an emphasis on medicinal
plants with antiulcerogenic, anti-inflammatory, antinociceptive and antidiarrheal
activity. She participates in the thematic project: “Standardized herbal medicines
for the treatment of chronic diseases”. During the Master’s degree, she worked with
animal models for gastroprotection. She currently works with animal models for
the evaluation of hormonal effects on gastric ulcer healing. Her current project is
titled: The role of the hydroalcoholic extract from the leaves of
Eugenia punicifolia
in
experimental peptic ulcer disease: characterization of anti-inflammatory, healing and
antiapoptotic mechanisms of action.
e:
larissaperico@hotmail.com