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allied
academies
J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
T
he present study is focused on investigating the anticancer
effect of
Pistacia atlantica
subspecies kurdica mastic gum resin
(MGR) on the proliferation of various cancerous and noncancerous
human cell lines exploiting MTT assay, microscopic evaluations, flow
cytometry, caspase activity and qPCR. The MTT results showed that
the mastic gum resin (0.01-100 μM) is selectively induced death
of cancer cells in a dose and time-dependent manner. Significant
suppression of proliferation of human cancerous cells was seen
with IC
50
value of 15.34±0.21, 11.52±0.18, 8.11±0.23 μg/mL and
5.2±0.8 μg/mL at 72 hours of treatment respectively for bile duct
cancer (cholangiocarcinoma) (KMBC), pancreatic carcinoma (PANC-
1), gastric adenocarcinoma (CRL-1739), breast adenocarcinoma
(MCF-7) and colonic adenocarcinoma (COLO205). On the other
hand, normal human colon fibroblasts (CCD-18Co) have not showed
adverse effect after treatment with various doses of a natural resin.
The study also showed that a natural resin at a concentration of
5.2±0.8 μg/mL for 72 h early signs of apoptosis as evidenced by
confocal microscopy imaging. Flow cytometry studies showed that
the natural resin significantly (P<0.05) arrests COLO205 cells at
G2/M phase of cell cycle. On the other hand, we revealed that, the
antiproliferative effect of natural resin on COLO205 cells is through
the apoptotic intrinsic pathway via activation of caspases -3 and -9.
In conclusion, the results revealed that natural resin can be further
developed as chemotherapeutic compound for the treatment of
cancers especially colon cancer.
e:
hemn.othman@univsul.edu.iqPistacia atlantica
subspecies kurdica mastic gum resin, a potent and novel natural plant metabolite for
cancer treatment
Hemn H O
1
, Heshu S R
1, 2
, Max S C
3
, Noordin M M
4
and
Hazilawati H
4
1
University of Sulaimani, Iraq
2
Komar University of Science and Technology, Northern Iraq
3
DigiCare, USA
4
Universiti Putra Malaysia, Malaysia