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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Lin28a expression protects against streptozotocin-induced β-cell destruction and prevents diabetes
in mice
Jain Jeong, Hyun Jin Kim
and
Myoung Ok Kim
Kyungpook National University, Korea
L
in28a is a highly conserved RNA-binding protein that
represses the miRNA let-7. Lin28a is highly expressed
in embryonic stem cells (ESCs) and is involved in ESC
differentiation. Lin28a also functions as a reprogramming
factorforinducedpluripotentstem(iPS)cells.Apreviousstudy
showed that Lin28a modulates glucose metabolism, insulin
sensitivity, and promotes cancer cell proliferation. Lin28a
overexpression enhances cell proliferation and facilitates
glucose transport in the mouse pancreatic β-cell line Min6.
To investigate the effect of Lin28a expression on β-cells,
cells were treated with the appropriate streptozotocin (STZ)
concentrations. Pancreatic β-cells overexpressing Lin28a
showed higher survival than mock cells. Furthermore, Lin28a
was found to promote proliferation and inhibit apoptosis in
STZ-treated cells. In addition, Lin28a-overexpressing cells
show enhanced glucose transport. Lin28a inhibits let-7
expression and activates the PI3K/Akt signaling pathway.
In addition, this study aimed to identify the relationship
between Lin28a and type 1 diabetes
in vivo
using Lin28a-
overexpressing transgenic (Tg) mice. Lin28a Tg mice showed
enhanced glucose transport and increased insulin secretion.
We performed STZ experiments to mimic diabetes
in vivo
.
Lin28a-overexpressing mice were found to have lower blood
glucose levels and higher survival following STZ treatment
of pancreatic β-cells. The islet of Langerhans in Lin28a-
overexpressing mice secretes more insulin than in WT mice
when subjected to STZ treatment. In conclusion, Lin28a
expression protects against STZ-induced pancreatic β-cell
destruction and promotes cell proliferation in pancreatic
β-cells. The results indicate that Lin28a improves the function
of the islet of Langerhans in mice.
Speaker Biography
Jain Jeong is currently studying for PhD at Kyungpook National University in Korea. His
current laboratory research work is focusing on elucidating gene function and their
relation to diseases using various transgenic mice models.
e:
j.jeong8700@gmail.com