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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Speciation of inorganic and organic selenium in human serum samples based on isopropyl
2-[(isopropoxycarbothiolyl) disulfanyl] ethane/ionic liquid by ultrasound-assisted dispersive liquid-
liquid microextraction
H Shirkhanloo
and
K N Merchant
Research Institute of Petroleum Industry, Iran
A
simple
in-vitro
speciation of inorganic selenium (SeIV
and SeVI) and organic selenium (Se-Cys, Se-T, Se-Alb
Se-M) in human biological samples based on isopropyl 2-
[(isopropoxycarbothiolyl) disulfanyl] ethane thioate (IICDET)
as a complexing agent were studied by ultrasound-assisted
dispersive liquid-liquid bio-microextraction (USA-DLLMBE).
In first stage, 100 μL (≈0.1 g) of hydrophobic ionic liquid of
C8MIM [PF6] were only added to organic selenium (Se-Cys,
Se-M, and Se-ALB) in 10 mL of standard solution and human
serum, urine and plasma samples that were thermally
extracted into IL phase at human biological pH in 10 min and
after dilution with 100 μL of acetone, directly determined by
electro thermal atomic absorption spectrometry (ET-AAS).
In second stage, after separation IL from sample, inorganic
selenium (Se IV) in remained samples was com plexed by
IICDET and extracted to IL at pH=4 (R-S2Se-R). After reduction
Se (VI) to Se (IV) by HCl with temperature 130
0
C, inorganic
Se speciation was obtained based on total Se determination.
After optimized conditions, the enrichment factor (EF),
Linear range and limit of detection (LOD) for inorganic
selenium were obtained 25.2, 0.02- 1.35 μg L-1 and 5 ng L-1
in human biological samples respectively. The validation of
methodology was achieved by certified reference material
(CRM) and ICP-MS.
e:
hamidshirkhanloo@yahoo.ca