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Journal of Clinical and Experimental Toxicology | Volume: 03 | ISSN: 2630-4570
alliedacademies
November 04-05, 2019 | Prague, Czech Republic
2nd World Congress on
TOXICOLOGY AND APPLIED PHARMACOLOGY
Notes:
Brazilian natural products as a promisingmedicine for the treatment of inflammatory
diseases and its nanoformulation
Luzia Kalyne Almeida Moreira Leal
Federal University of Ceará, Brazil
During the development of new bioactive materials as
tools for therapeutic use, one of the key questions is
to understand how they interact with biological systems.
In this way, it is essential to describe the mechanism of
action aiming to understand how these materials induce
their pharmacological effects and if these actions may
cause health risks. New materials such as nanoparticles
have attracted the attention of academy and industry.
In particular, in Brazil we have researched several plant
extracts and free or nanoencapsulated molecules for the
treatment of inflammatory diseases using experimental
models in vitroand in vivo. We have investigated the anti-
inflammatory effect and toxicity of plant products, including
imidazole alkaloids (Epiisopiloturine (EPI) and epiisopilosine
(EPIIS) from Pilocarpus microphyllus Stapf), eugenol (a
free or nanoencapsulated terpene) and dry extract of
Amburana cearensis (microparticles). The EPI and EPIIS are
side products in the Brazilian pharmaceutical industry which
showed anti-inflammatory and antioxidant activities. Both
alkaloids inhibited the degranulation of activated human
neutrophils. This effect was accompanied by the reduction
of ROS, the prevention of the increase of intracellular Ca2+
and decrease of the density of cytosolic NF-kB, and inhibition
of TNF-α and IL-6 production. The EPI and EPIIS also inhibited
carrageenan-induced inflammatory hypernociception in
mice and reduced myeloperoxidase (MPO) levels. Eugenol
(EUG) is a terpene present in essential oils of plants which
has attracted attention due to its anti-inflammatory
properties, as well as antioxidant effect. Despite of these
pharmacological properties it presents irritant effect on skin
which limit its use in topic, suchas for treatment of dermatitis.
To overcome it, we developed eugenol- loaded polymeric
nanocapsules. The EUG, inhibited the ROS production in
human neutrophil, but it was toxic in human keratinocyte
and did not interfere with ear edema induced by TPA inmice.
However, the nanocapsules of EUG (NCEUG) prevented its
cytotoxicity in keratinocytes, and reduced ear thickness
of mice (experimental model of dermatitis) reducing the
MPO activity and the concentrations of IL-6 and KC (CXCL
1). Together, these results showed that NCEUG promoted
a reduction in cytotoxicity of EUG and improved its anti-
inflammatory effect. Parkinson’s and Alzheimer’s Disease are
neurodegenerative diseases which neuroinflammation has
an important role. Microglia is part of the innate immunity
of central nervous system, being it activation one of the main
mechanisms of inflammation responses. The standardized
dry extract of A. cearensis (actives markers: coumarin and
amburoside) reduced LPS-stimulated nitrite release on
microglial and reduced the expression of iNOS (Western
blot analysis). These findings suggest that molecules and/
or plant extract from Brazilian medicinal plants, and its
nanoencapsulation possess promising anti-inflammatory
potential acting through the modulation of inflammatory
response appear non-toxic.
Biography
Luzia Kalyne Almeida Moreira Leal is pharmacist and received her PhD
in Pharmacology from the Federal University of Ceará, Brazil in 2006.
She is Pharmacognosy professor at Federal University of Ceará since
1996. Her research interests are in Pharmacognosy and Pharmacology
of Natural Products for the development of new medicines to treat
inflammatory diseases. She is founder and coordinator of the Center of
Pharmaceutical and Cosmetics Studies.
e:
Luzia Kalyne Almeida Moreira Leal, J Clin Exp Tox, Volume: 03
DOI: 10.35841/2630-4570-C2-009