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Journal of Biomedical Research | ISSN: 0976-1683 | Volume 30
allied
academies
March 14-15, 2019 | London, UK
T issue Engineer ing, Stem Cel ls and Regenerat ive Medicine
Cel l and Gene Therapy
World Congress on
International Conference on
&
Joint Event
T
he spermatogonial stem cells (SSCs) are the only adult
stem cells that are responsible for the transmission
of genetic information from an individual to the next
generation. SSCs play a very important role in the
maintenance of normal tissue and provide an understanding
of the rudimentary reproductive biology of gametes and a
strategy for diagnosis and treatment of infertility and male
reproductive toxicology. Androgens / oestrogens are very
important for the suitable maintenance of male germ cells.
There is also evidence confirming the damaging effects of
oestrogen-like compounds on male reproductive health.
We investigated the effects
in vitro
, of DES on mouse
spermatogonial stemcells separated using staput unit-gravity
velocity sedimentation, evaluating any DNA damage using
the comet assay and apoptotic cells detected by the TUNEL
assay. Immunocytochemistry assays showed that the purity
of isolated mouse spermatogonial cells were 90%, and the
viability of this isolated cell was over 96%. The intracellular
superoxide anion production in SSCs was detected using the
p-Nitro Blue Tetrazolium (NBT) assay. The viability of cells
after DES treatment was examined by CCK8 (cell counting
kit-8) assay. The results showed DES-induced DNA damage
causes an increase in the intracellular superoxide anion.
Investigating the mechanism and biology of SSCs not only
delivers a better understanding of spermatogonial stem cell
regulation but ultimately would also be a new target for
male infertility and testicular cancers.
S
peaker Biography
Diana Anderson holds the established chair in Biomedical Sciences at the University of
Bradford. She obtained her first degree in the University of Wales and second degrees
in the faculty of medicine, University of Manchester. She has 450+ peer-reviewed
papers, 9 books, has successfully supervised 32 PhDs, is an editorial board member of 10
international journals. She is Editor-in-Chief of a book series on Toxicology for the Royal
Society of Chemistry. She gives plenary and key note addresses at various international
meetings. She is a consultant for many international organizations, including WHO, EU,
NATO, TWAS, UNIDO, OECD.
e:
d.anderson1@bradford.ac.ukDiana Anderson
University of Bradford, UK
Induced oxidative DNA damage in spermatogonial stem cells by diethylstilbestrol
in vitro
Diana Anderson
, Biomed Res, Volume 30
DOI: 10.4066/biomedicalresearch-C1-024