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Biomedical Research
|
Volume 29
Page 37
Note:
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
PLATELET LYSATE INDUCES
CHONDROGENIC DIFFERENTIATION
OF UMBILICAL CORD DERIVED
MESENCHYMAL STEM CELLS
Ghmkin Hassan
Okayama University, Japan
Purpose:
Articular cartilage has poor repair ability and still confers a
major challenge in orthopedics. Mesenchymal stem cells (MSCs) are
multipotent stem cells with potential to differentiate into chondrocytes
in the presence of transforming growth factor beta (TGF-β). Relevantly,
platelet lysate (PL) contains many growth factors including TGF-β and
ameliorates cartilage repair. Thus, we investigated the ability of PL to
direct chondrogenic differentiation of MSCs along with other standard
differentiation components in pellets culture system.
Methods:
We isolated and expanded MSCs from human umbilical
cords using PL supplement medium and characterized cells by
immunophenotyping and differentiation potential to adipocytes and
osteocytes. We further cultured MSCs as pellets in chondrogenic
differentiation medium supplemented with PL. After 21 days, pellets
were processed for histological analysis and stained with alician blue and
acridine orange. Expression of SOX9 was investigated by RT-PCR.
Results:
Although MSCs maintained their stemness characteristics
in PL supplement medium, distribution of cells in pellets cultured in
chondrogenic differentiation medium supplemented with PL was more
similar to cartilage tissue-derived chondrocytes than negative control.
In addition, intense alician blue staining indicated increased production
of mucopolysaccharides in differentiated pellets, which also showed
elevated expression of SOX9 detected by RT-PCR.
Conclusions:
Our data suggest that MSCs could be differentiated to
chondrocytes in the presence of PL and absence of exogenous TGF- β.
Further research needs to be conducted to understand the exact potential
role of PL in chondrogenic differentiation and chondrocyte regeneration.
Ghmkin Hassan obtained his master degree
in biotechnology and molecular biology from
Damascus University, Syria. Presently, he is a
research scholar at Okayama University, japan.
He was interested in isolation and optimizing
stem cell culture conditions which was a part
of “mesenchymal stem cell optimization culture
conditions” in national commission for biotech-
nology Syria and his recent focus is on origin of
cancer stem cells and its microenvironment.
hsn.ghmkin@gmail.comBIOGRAPHY
Ghmkin Hassan, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-005