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Biomedical Research
|
Volume 29
Page 36
Note:
allied
academies
CARDIOLOGY AND CARDIOVASCULAR MEDICINE
STEM CELLS AND REGENERATIVE MEDICINE
&
International Conference on
International Conference on
J u n e 1 8 - 1 9 , 2 0 1 8 | O s a k a , J a p a n
Joint Event on
THE ROLE OF HEMATOPOIETIC
CELL-DERIVED MULTIPOTENT STEM
CELLS IN SKIN TISSUE REPAIR AND
REGENERATION
Yunyuan Li
University of British Columbia, Canada
N
on-healing and chronic wounds cause tremendous suffering and
debilitation .To look for new strategies to improve healing of damage
skin tissue and understand the mechanism underlining a complex wound
healing process, in last a few years, we have conducted a serial of studies
to identify healing promoting factors. We found that the macrophage
colony-stimulating factor (M-CSF) released from proliferating skin cells
could induce a subset of hematopoietic cells to be dedifferentiated into
multipotent stem cells. Interestingly, hematopoietic cell-derived, stage
specific embryonic antigen (SSEA)-1 and-3 positive, multipotent stem
cells were transiently present in the wound site after skin injury. In this
study, we further explored the roles of these hematopoietic cell-derived
multipotent stem cells to repair injured skin and hair regeneration in a
mouse model. Hematopoietic cell-derived multipotent stem cells were
generated by culture mouse splenocytes in a medium containing M-CSF.
Skin excisional wounds were generated by punch biopsy in mice which
received nothing (control) or one million splenocyte-derived stem cells
by intra-dermal or tail vein injection simultaneously. Injected stem cells
expressing GFP or labeled with a fluorescent dye Dil were used for cell
tracing. Immunofluorescent staining was used to identify the cell source
in healing skin tissue. Results revealed that addition of M-CSF or its
antibody to increase or reduce the number of hematopoietic cell-derived
stem cells at the wound site could accelerate or slow skin wound healing
in mice. We also demonstrated that injected hematopoietic cell-derived
stem cells could be differentiated into fibroblasts, keratinocytes and
blood vessel-like structures
in vivo
. These blood cell-derived skin cells
were the major contributions of healing skin. Furthermore, our results
suggested that hematopoietic cell-derived multipotent stem cells could
participate in new hair follicle regeneration. In conclusion, hematopoietic
cells are the major contributions and cell source for skin tissue repair and
hair regeneration.
Yunyuan Li has completed his PhD in 2005
from the University of Alberta, Canada. He is a
senior researcher at Burn and Wound Healing
Research Laboratory in the University of British
Columbia. He has published over 40 papers.
Recently, his research interests were focused
on the roles of hematopoietic cell-derived mul-
tipotent stem cells in skin wound healing, hair
follicle regeneration and inflammation.
yunyuan.li@ubc.caBIOGRAPHY
Yunyuan Li, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C2-005