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Journal of Environmental Waste Management and Recycling | Volume 1
March 05-06, 2018 | London, UK
Recycling & Waste Management
5
th
International Conference on
C
ellulolytic bacteria have a bio-activating role in the
composting process. A study was carried out to isolate
and identify cellulolytic bacteria from various sources. The
isolates were cultured in the carboxymethyl cellulose(CMC)
agar medium and incubated at 30°C for 3–7 days. Based
on morphological characteristics of the isolates, maximum
diameter of a clear zone around the colony and maximum
cellulolytic activity, eight isolate were selected for further
studies regarding composting experiments. Molecular
tests based on PCR amplification and sequencing of 16S
rRNA gene of isolates showed the closest phylogenetic
similarity with the species of Stenotrophomonas rhizophila
DSM14405 (99.8%), Brevibacterium halotolerans DSM8802
(99.6%), Achromobacter marplatensis B2 (99.8%), Bacillus
methylotrophicus
CBMB205
(100%),
Pseudomonas
azotoformans IAM 1603 (99.7%), Bacillus sonorensis NBRC
101234 (99.8%),Bacillus subtilis KCTC 13429 (100%) and
Ochrobactrum thiophenivorans DSM 7216 (99.3%). The
study of the isolates impact on the composting of palm
wastes in a randomized complete block design with 11
treatments in 3 replications showed that strain IB (B.
methylotrophicus) caused a significant decrease in C:N ratio
(58%). The increasing of microbial respiration compared with
control after 30 days incubation at 37°C showed that the B.
methylotrophicus strain IB with cellulolytic characteristics
can be applied to hydrolysis of cellulosic biomass in the
composting processes.
e:
hosaffary@yahoo.comStudy of effectiveness of cellulolytic bacteria from different sources in bio-activating of composting
process
Hossein Saffari
Agricultural Research Education and Extension Organization (AREEO), Iran