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Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Phytochemical composition and biological properties of a hydroalcoholic extract obtained from the aerial
parts of Matthiola incana (L.) R. Br. (Brassicaceae) growing in Sicily
Maria Fernanda Taviano1, Emilia Cavo2,1, Andreana Marino1, Salvatore Ragusa3, Paola Dugo1, Francesco Cacciola4, Pierangela Irrera2,1,
Concetta Condurso1, Maria Merlino5 and Natalizia Miceli1
1Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Italy
2Foundation Prof. Antonio Imbesi, University of Messina, Italy
3Magna Graecia University of Catanzaro, Italy
4Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Italy
5Department of Veterinary Sciences, University of Messina, Italy
In continuation of our studies on species belonging to the
Brassicaceae family growing in Sicily (Italy), this work aimed to
characterize the phenolic profile and the volatile constituents
and to evaluate some biological activities of a hydroalcoholic
extract (80% methanol) obtained from the aerial parts (leaves
and flower buds) of Matthiola incana (L.) R. Br. grown wild
around Capo D’Orlando (Messina, Sicily) The HPLC-PDA/
ESI-MS analysis led to the identification of twelve phenolic
compounds, two phenolic acid derivatives (5.46 mg/g extract)
and ten flavonoids (155.85 mg/g extract), being luteolin-
glucoside the most abundant component (57.07 mg/g ± 0.87%
RSD). By SPME/GC-MS fifty-one volatile constituents were fully
characterized, and (Z)-9-Octadecen-1-ol turned out to be the
most abundant one (24.35%). The antioxidant potential of M.
incana extract was evaluated by in vitro methods based on
different approaches and mechanisms: DPPH, reducing power,
and ferrous ions chelating activity assays. The extract exhibited
mild activity both in the DPPH and reducing power assays,
whereas it was found to possess good chelating properties,
reaching approximately 90% activity at the highest tested dose
(2 mg/mL). In order to investigate the antioxidant efficacy of
M. incana extract in a biological setting, the ability to protect
bacterial growth and survival from the oxidative stress induced
by hydrogen peroxide (H2O2) was evaluated on Escherichia coli.
The extract displayed protective effect against H2O2-induced
oxidativedamage. Theantimicrobial activityofM. incana extract
against selected bacteria and yeasts were assayed by standard
methods. The extract didn’t show activity against the tested
strains (MICs > 500 µg/mL). Finally, the potential toxicity was
investigated using Artemia salina lethality bioassay. Themedian
lethal concentration value indicated that the extract did not
display any toxicity against brine shrimps (LC50 > 1000 µg/mL).
Speaker Biography
Maria Fernanda Taviano obtained the following academic qualifications at the Faculty of
Pharmacy of the University of Messina (Italy). Specialization degree in Pharmacognosy;
PhD in Pharmacognosy; 1st level Master degree in Applied Biotechnology. She has been
research grant holder for a period of four years. Currently, she is RTD-A Researcher (SSD
BIO/15-PharmaceuticalBiology)attheDepartmentofChemical,Biological,Pharmaceutical
and Environmental Sciences of the University of Messina. She is Editorial Board Member
of the scientific international journal “Pharmacognosy Magazine”. Her scientific research
activity is mainly focused on the phytochemical study and the evaluation of “in vitro” and
“in vivo” biological properties of active products from plant sources, obtainable also by
means of “in vitro” culture systems.
e:
Maria Fernanda Taviano, Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-002