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allied
academies
Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
In vitro
propagation of native plant species from Kuwait desert
Suad Almazrooei
Kuwait University, Kuwait
C
lonal propagation by tissue culture of selected plant species,
Native to Kuwait desert, was studied. Micropropagation of
Haplophyllum tuberculatum, Farsetia aegyptica, Heliotropium
bacciferum, Ochradenus baccatus, Rumex vesicarius, Salvia
spinosa
and
Acacia pachyceras
through axillary bud culture
showed different morphogenic potentials depending on
species type and ratio of 1-naphthylacetic acid (NAA) to
6-benzylaminopurine (BAP) supplemented in Murashige and
Skoog (MS) culture medium. Production of adventitious shoots
fromcallus was the common and frequent response in all tested
species. This was mostly achieved at low concentration of NAA
within the NAA and BAP combinations applied. Induction of
embryogenic callus in
Acacia pachyceras
var.
najdensis
was
achieved from embryo axes explants. The largest callus mass
was initiated on MS medium supplemented with 0.1 mg/l
2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l BAP.
Regeneration of new plantlets from the induced embryogenic
callus was achieved on MS medium supplemented with
BAP only. In contrast, initiation of embryogenic callus from
leaf explants of
A. pachyceras
was achieved on MS medium
supplemented with 0.5 mg/l BAP in combination with 0.5
mg/l or 0.1 mg/l 2,4-D. This is the first report of somatic
embryogenesis in
A. pachyceras
.
Speaker Biography
Suad Almazrooei is currently working as a Professor in Kuwait University. He is in
the department of Biological Sciences. He has completed several projects and has
expertise in the field of conservation genetics and microsatellites
e:
s.almazrooei@ku.edu.kwSuad Almazrooei
, Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-002