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Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Fingerprinting of different cultivars of Banana Musa sp L using microsatellite DNA marker
S R Mulla
University of Horticultural Sciences, India
Banana is an important crop grown worldwide and is one of
the most important food crops after maize, rice, wheat and
cassava globally. Despite the importance of the crop, production
is threatened by various constraints such as pests and by a
multitude of serious bacterial, viral and fungal diseases. Banana
breeding programs are currently focused on the introgression
of diverse traits that range from disease resistance/tolerance
to yield and fruit quality. Molecular genetic studies are of
fundamental importance for increasing our knowledge base
and resources for accelerated genetic improvement of the
Banana, by allowing theanalysis of genetic diversity. Thepresent
investigationwasdemonstratedthepotentialuseofSSRmarkers
for assessment of genetic diversity and relationship among forty
ecotypes of four genotypes of Banana (Elakki, Rajapuri, Red
Banana and Rasabale). Suckers were collected from different
geographical regions of southernpart of India (Karnataka, Kerala
and Tamil Nadu). In order to see the inter-relationship among
the Banana ecotypes, a phylogenetic tree was constructed
from the pairwise distance matrices. Genetic diversity of
Banana genotypes was analyzed using Darwin’s software with
10,000 boot straps. The dendrogram based on UPGMA cluster
analysis separated the genotypes into four major clusters but
the distinctiveness between the ecotypes was not observed.
The cluster I consisted of all the Elakki ecotypes, whereas,
Rajapuri ecotypes were located in cluster II, and Red banana
ecotypes were located in cluster III and cluster IV consisted
of Rasabale ecotypes. This revealed that there was close
relatedness between ecotypes, which could not differentiate
them irrespective of their different places of origin and
utilization of less number of primers for screening the ecotypes.
Speaker Biography
S R Mulla did his Ph.D. from University of Agricultural Sciences, Bengaluru, India. He
is presently serving as Assistant Professor, Department of Biotechnology and crop
improvement, University of Horticultural Sciences, Bagalkot, India. He has published more
than 10 papers in reputed journals.
e:
S R Mulla, Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-002