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Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Electrical stimulation of Arabidopsis thaliana
Diane Krill
Point Park University, USA
Plants respond to their environment in a multitude of
ways. In our first report, we described a rapid movement
response that occurred in plants without any physical contact
with the seedlings based on electric charge. Experiments with
genetically altered seedlings followed to isolate the pathway
required for movement. Three varieties of seeds were planted
in the laboratory under sterile conditions. After 5-10 days the
seedlings were tested for their response to electric field stimuli,
and video responses were recorded with the fluctuation in
the electric field measured with an oscilloscope. Mutants of
A. thaliana from ABRC tested included Jasmonate Resistant
1 (JAR1), which lack a functional synthetase in the jasmonate
signalling pathway, andNHX7/SOS1, which containhomozygous
mutagenized alleles for the Na+/H+ antiporter. Wild type (wt)
( Col-1) from ABRC and mutant seeds were grown on low K+
media to support the growth of the hypersensitive NHX7/SOS1.
Plant seedlings of wt Thymus vulgaris, Arabidopsis thaliana and
Mentha spicata, starting at the 2-3 leaf stage, were capable of
msec movement responses to objects that conveyed an electric
charge. Movement responses were observed in 50% of wt Col-
1 seedlings plated in low K+ media compared to 87% response
of wt in regular salt media. The response rate was 18% for
NHX7SOS1 and 24% for JAR1. Both types of genetically altered
seedlings had a lower level of responsiveness compared towt A.
thaliana. Reduced responsiveness in JAR1 and SOS1 seedlings
may be the result of seed genotype mixing, or crossover
required between signalling pathways.
Speaker Biography
Diane Krill completed her Ph.D. in Developmental & Molecular Biology from Case
Western Reserve University in Cleveland, Ohio. Her postdoctoral studies and an M.P.H.
were completed at the University of Pittsburgh Graduate School of Public Health.
She is currently a professor of biology at Point Park University in Pittsburgh, USA.
The majority of her research publications involve the tumour microenvironment and
angiogenesis as it relates to cancer. She successfully isolated a plant compound with
therapeutic potential that prevents new blood vessel development in vivo in zebrafish,
and in human stem cells. The plant assay system used to establish the effects of the
plant compound on vascular tissue led to the study of electrical stimulation in the
plant model, Arabidopsis thaliana . She is a member of the American Association for
Cancer Research, the MS Society, and serves as a reviewer for the Journal of Cancer &
Nutrition, Ethnopharmacology and other journals.
e:
Diane Krill, Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-002