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allied
academies
Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Antidiabetic and toxic evaluation of polar extracts of
Myrciaria dubia, Coussapoa asperifolia
and
Remigia pedunculata
Victor Sotero
National University Intercultural of the Amazon (UNIA), Peru
T
he aimof this work is to evaluate three plant species in order
to observe their behavior as antidiabetic with the polar
extracts of
Myrciaria dubia
(fruit),
Coussapoa asperifolia
(bark)
and
Remigia pedunculata
(leaf), native to the Peruvian Amazon,
were evaluated in terms of their antidiabetic activity (AAD),
and toxic (ACT) in vitro and in vivo, according to the following
methodology: a) AAD to inhibit the α-glucosidase, and b) ACT,
evaluate the dose lethal in front of
Artemia franciscana
. For the
in vivo assays, the AAD and ACT assays were performed, for
the first, the extracts were administered to 10 Albino mice and
diabetics (induced with streptozotocin), in concentrations of
100, 500 and 1000mg/kg, for one hour, evaluating the glycemia
index, and acute toxicity test was performed, administering to
the mice a concentration of 2000 mg/kg of the extracts under
14 days. In addition, analyzes of the main chemical families
were carried out using UV/Vis spectroscopy for phenolic
compounds, alkaloids and saponins of the methanolic extracts
of the three species. According to the results, these species
have an important antidiabetic activity, obtaining a percentage
of inhibition against α-glucosidase in extracts of 500 μg/ml of
51.5%, 40.7% and 97.4%, for
M. dubia
,
R. pedunculata
and
C.
asperifolia
respectively. Thus, according to the results of acute
toxicity, they did not present some toxicological problems in
certain organs and the lowest glycemic indexes in diabetic mice
was in the concentration of 1000 mg/kg where there were
obtained: 92.6, 96.8 and 96.8 mg/dl, for
M. dubia, C. asperifolia
and
R. pedunculata
respectively. The highest concentration in
secondary metabolites were the phenolic compounds being
794.1 mg/g in
M. dubia
; 324.7 mg/g in
R. pedunculata
and
214.5 mg/g in
C. asperifolia
.
e:
pproyectopalmeras@gmail.comPlant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-003