5 / 25
Page 42
allied
academies
Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
The early expression of
WUSCHEL
, an organ identity gene, is a marker in Tobacco and
Beta palonga
during the onset of
in vitro
shoot morphogenesis
Gaurab Gangopadhyay
and
Marufa Sultana
Bose Institute, India
T
he two in vitro morphogenesis pathways leading to whole
plant regeneration involve either shoot organogenesis
followedby root organogenesis or somatic embryogenesis. Both
developmental pathways can occur either directly without an
intermediate callus stage, or indirectly following an unorganized
callus stage. Reports of involvement of
WUS
gene concerning
in vitro
shoot organogenesis are very scanty, particularly in the
non-model plant systems, i.e., plants other than
Arabidopsis
.
We have studied the role of growth regulators behind
in vitro
shoot organogenesis and somatic embryogenesis in two
plant systems, viz. tobacco (
Nicotiana tabacum
L. var. Jayasri)
and
Beta palonga
R.K.Basu & K.K.Mukh, a model and a non-
model plant system respectively. We have also correlated the
phenomena of de differentiation with the relative expression
of
WUS
(
WUSCHEL
) gene in a time-dependent manner. The
results indicated that early
WUS
gene expression is a definite
marker for
in vitro
shoot organogenesis in tobacco and
Beta
both in direct and indirect modes of regeneration. Additionally,
we have performed a comparative homology modelling and
in
silico
structural analysis of
WUSCHEL
proteins of
B. palonga
,
B. vulgaris
, and
Arabidopsis
to find out the commonality of
the ligand binding site. The amino acids of the binding sites
were identical (Arginine, Tryptophan, Proline, Asparagine,
and Tyrosine) in the three materials under study; except two
additional amino acids (Isoleucine and Alanine) in
B. vulgaris
.
Speaker Biography
Gaurab Gangopadhyay, Associate Professor, Division of Plant Biology, Bose Institute,
Kolkata, India is in the field of plant science research for last 28 years. After graduating from
Presidency College, Kolkata and post graduation in Botany (University of Calcutta) he did his
doctoral work at Bose Institute. He has a Post Doctoral Research experience for over twelve
years. Dr Gangopadhyay has 68 research publications and 58 NCBI GenBank submissions.
His H-index is 14, and he acts as editorial board member and reviewer for peer-reviewed
scientific journals. His present area of research interest is Marker Assisted Plant Breeding,
Plant Biotechnology and Molecular Biology.
e:
gaurab@jcbose.ac.inGaurab Gangopadhyay et al.
, Plant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-003