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Journal of Agricultural Science and Botany | Volume: 2
November 15-16, 2018 | Paris, France
Plant Science
Natural Products,Medicinal Plants and Traditional Medicines
International Conference on
Joint Event
&
Evaluation of the antioxidant activity of 31 Amazonian species
Dora Garcia
National University of Peruvian Amazon (UNAP), Peru
T
he Amazon has an interesting reserve of phytotherapeutic
resources and ancestral form have been used by the natives
of this region in the cure of various diseases, and reported in
several ethnobotanical studies. The objective of this work
is to evaluate the antioxidant capacity and the content of
phenols and total alkaloids in the methanolic extracts of the
leaves of 31 plant species collected in 2015 in the town of
Tamshiyacu, - Loreto Region – Peru. The percentage of free
radical inhibition of DPPH were calculated, in order to select all
three with high activity. From these, the total concentration of
phenolic compounds and alkaloids were analyzed by UV / Vis
spectrophotometry.Theextractsweresubjectedtofractionation
in a chromatographic column and the fractions with similar
molecules, grouped using thin layer chromatography. The final
fractions were analyzed by GC-Ms to identify the molecules
present in them. About the results, the species that showed
the best activity at concentrations lower than 5.0 mg / ml,
were
Virola sebifera, Caryocar glabrum
and
Tapirira guianensis
.
The concentration of total phenolic compounds was 18580.9,
15180.7 and 11568.7 mg / 100g for
V. sebifra, C. glabrum
and
T. guiannensis
, and total alkaloids were 36.6, 0.0 and 74.0 mg /
100g for these same species. The main secondary metabolites
present are3,5-di-tert-butyl-4-hydroxyanisole andnormetadhol
and caryophyllene in
V. sebifera
, diisooctyl dicarboxylate.1,2-
benzene and3,5-bis (1,1-dimetyl ethyl-phenol in
C. glabrum
and
diisoctylphthalate,α-panasinseno,andvitaminEin
T.guianensis.
Of the 31 species under study, three were found with high
antioxidant activity and in which they emphasized their high
concentration of phenolic compounds, inferring in a certain
sense, that these substances are the cause of this activity.
e:
doegato@hotmail.comPlant science & Natural Medicine 2018, Volume 2
DOI: 10.4066/2591-7897-C1-003