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allied
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September 20-22, 2017 | Toronto, Canada
10
TH
AMERICAN PEDIATRICS HEALTHCARE &
PEDIATRIC INFECTIOUS DISEASES CONGRESS
Pediatric Healthcare & Pediatric Infections 2017
Background:
Staphylococcus aureus
, particularly the
MRSA strains (resistant to oxacillin/methicillin and other
antimicrobials) causes infections commonly including
pneumonia accounting for 3-5%, globally, Since childhood-
pneumonia in Malaysia ranges between 7-44% but,
molecular epidemiology of naso-pharyngeal
S. aureus
has
been scarcely reported, we conducted this cross-sectional
study.
Objective(s):
To determine prevalence of
staphylococcal
pneumonia
among under-5 years-old and to compare
phenotypic and genotypic diversities between oxacillin/
methicillin sensitive (MSSA) and resistant (MRSA)
S. aureus
-
strains.
Methodology:
With mother’s consent, nasopharyngeal-
swabs (NPS) were collected from randomly selected 220
hospital admitted children in two tertiary-care hospitals
in Kedah, Malaysia. Bacterial isolates grown on mannitol-
salt and blood-agar plates were incubated (+35o-37oC),
overnight. Colony morphology read, gram-stained done
and bio-chemical tests (+ve catalase & coagulase & CHO-
fermented) performed. Antimicrobial-resistance using 8
antibiotic-disks: AMC
20
, CRO
30
, CIP
5
, E
15
, CN
10
, S
10
, TE
30
, VA
30
;
OX
1
) were performed. For genetic-analysis PCR (
Sigma
, USA)
were performed employing rapid DNA isolation and thermal
cycler (
Bio-Rad
, USA) using two specific-primers
fem
A
(confirming
MSSA
) and mecA (confirming
MRSA
) by tracing
electrophoretic-DNA band-size on agarose-gel.
Results:
Of all suspected cases, 76%were clinically diagnosed
as pneumonia. NPS from 32.6% yielded MSSA among which
39.4% were MRSA when identified phenotypically. Lower
MRSA-prevalence was observed genotypically (32%) than
phenotypically (39%). While no differences existed between
MRSA & MSSA phenotypically, genetically it did significantly
(p<0.01), particularly for pneumonic cases (p<0.04) showing
marked difference in carrying
fem
A
and
mec
A
genes (p<0.00).
Conclusion and Recommendation: Although MSSA, other
than MRSA is associated to cause childhood-pneumonia,
detailed molecular-epidemiology is recommended to
elucidate genetic diversities of
S. aureus
implicating
childhood pneumonia.
e:
kselim2256@gmail.comPneumonia and lower respiratory infections among under-5 hospitalized children in malaysia: Insights
on nasopharyngeal staphylococcus aureus yielding phenotypic and genotypic variations between
oxacillin/methicillin sensitive (MSSA) and resistant (MRSA)-strains
Kazi Selim Anwar
1
, Tetsuya Matsumoto
2
, Rayhan Ahmed
3
and
Anwar Hossain4
1
AIMST University, Malaysia
2, 4
Tokyo Medical University, Japan