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Journal of Clinical Pathology and Laboratory Medicine | Volume 3
July 05-06, 2019 | Paris, France
Pathology and Surgical Pathology
2
nd
International Conference on
AnnexinA7 knockdown could suppress cell proliferation and metastasis in gastric cancer
Xin Li
Chengde Medical University, China
G
astric cancer is one of the most deadly disease around
the world. However, the mechanism of it is still unclear.
AnnexinA7 is one of the calcium-dependent phospholipid
binding protein which is involved in the cell membrane
dynamics and cell signal transduction. Whether AnnexinA7
play any role in the development of gastric cancer is still
unknown. In this study, RNA interfering was performed to
silence AnnexinA7 in gastric cancer cell lines MGC-803 and
MKN-45. Cell proliferation was detected by MTT method,
while cell metastasis was examined by wound healing assay
and trans well assay respectively. Western blotting was
employed to detect the expression of the EMT marker genes
and the key genes in the ERK signal pathway. Our results
indicated that AnnexinA7 acted as an oncogene suppressor
and could suppress cell proliferation and metastasis in gastric
cancer which might via ERK signal pathway.
Speaker Biography
Xin Li has completed her PhD at the age of 36 years from Hebei Medical
University, China. She is the professor of ChengdeMedical University, China.
e:
hbcdlx@cdmc.edu.cn