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February 18-19, 2019 | Paris, France

Joint Event

Green Chemistry and Technology

World Congress on

International Conference on

Organic and Inorganic Chemistry

Journal of Industrial and Environmental Chemistry| Volume: 3

Study on

Mentha Pulegium

L. from M’rirt (Morocco): Antibacterial and antifungal activities of a

pulegone-rich essential oil

Amalich Smail

University Moulay Ismail, Morocco

ennyroyal or

MenthaPulegiumL

. is a species fromLamiaceae

family which is extremely rich in aromatic essence.

This species is used for various purposes. In the present work,

we have carried out a study on chemical composition and

evaluation of antimicrobial activity and antifungal activity of its

essential oil. To achieve this aim, essential oil was first extracted

by hydrodistillation from bloomy tops of

Mentha pulegium L

harvested in the wild during the month of July 2014 in M’rirt.

This extraction yielded about 5.2 ± 0.25% of essential oils (EOs).

Then, EO was analyzed by gas chromatography coupled with

mass spectrometry (GC-MS).

Results of this analysis showed that it contains two major

compounds: the first one is “pulegone” and the second is

“piperitenone”. Both, these molecules represent 98.01% of

the whole identified compounds. Antibacterial activity of this

EO was assessed against four bacterial strains by disc-diffusion

method on agar medium and macrodilution method in liquid

medium. Results showed that EO of

Mentha pulegium L

. has a

very significant antibacterial activity in a liquidmedium towards

Escherichla Coli

and

Staphylococcus Aureus

with respectively

1.4 µl/ml and 2.8µl/ml as minimal inhibitory concentrations.

Evaluation of antifungal activity revealed that this essential

oil is able inhibit mycelial growth as well as sporulation of the

three fungal species tested (

Aspergillus Sp., Penicillium Sp

. and

Rhizopus Sp

.) at low concentrations. In conclusion, essential

oil of pennyroyal was very active. Indeed, it was endowed with

a relatively interesting antibacterial activity and an excellent

inhibitory potency on mycelial growth and sporulation of the

tested fungi. Inhibitory effect of this essential oil suggests

prospects of application in the field of foodstuffs conservation

:malich80@gmail.com

J Ind Environ Chem 2019, Volume 3

DOI: 10.4066/2591-7331-C1-009