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J Med Oncl Ther 2017 | Volume 2 Issue 4
Oncology and Biomarkers Summit
November 27-28, 2017 | Atlanta, USA
Annual Congress on
Development of a novel DNA bio-marker for the qualitative and quantitative detection of malayan
box turtle (
Cuora amboinensis
) material in traditional chinese medicines
Asing
University of Malaya, Malaysia
M
alayan box turtle (
Cuora amboinensis
) (MBT) is a
protected species inMalaysia since 2005 and prohibited
(haram) animal species in Muslim foods and medicines. The
widespread availability of commercial traditional Chinese
medicines across Malaysia may offer the opportunity of
turtle product trafficking under the covert of halal brands,
needing to develop a convenient and reliable method both
for the qualitative and quantitative tracing of turtle materials
in medicines. Several polymerase chain reaction (PCR) assays
have been proposed for the detection of MBT species under
various routes but they are based on long-length targets
which break down under the state of decomposition, making
them unsuitable for the forensic detection in medicines and
other potential routes. To overcome this knowledge gap, for
the first time, we developed a short length DNA target for
the quantitative detection of MBT tissues by SYBR green real-
time PCR systems. The assay specificity was checked against
20 different species and DNA biomarker stability was tested
under various meat tissue processing conditions, including
boiling, autoclaving and micro oven heating under pure and
admixed matrices. The limit of detection (LOD) of the SYBR
green duplex real time PCR system was 0.00001 ng DNA and
0.001% (w/w) MBT meat under mixed matrices. Finally, 120
traditional Chinese medicines samples were surveyed by
SYBR green duplex real time PCR system and 23% of them
were found to be MBT-positive (0.00157 to 0.0612 ng/µL),
respectively. Thus the methods were suitable for real-world
application and they confirmed the widespread speculation
that MBT materials are widely used in Chinese medicines and
herbal medicines as well as this technique could be applied
medical diagnosis science.
Speaker Biography
Asing has completed his PhD in Biology and Biochemistry under the supervisor of
Md. Eaqub Ali, Associate Professor, at Nanotechnology and Catalysis Research Centre,
University of Malaya, Kuala Lumpur, Malaysia. He has obtained his MS degree in
Biochemistry and Molecular Biology under the supervisor of Professor Dwaipayan
Sikdar, University of Chittagong, Bangladesh. His research interests are on DNA markers
development, Biochemistry, Molecular Biology, Food Science and Pharmaceutical
Science. He has contributed and published 17 research articles in top rating research
journals. He has 5 conference proceedings and presented oral (3) and poster (2) in
prestigious international conferences in Malaysia, Indonesia, Thailand and Singapore
respectively. Before being a PhD student, he had worked as research assistant, quality
control office in leading Biochemistry and Molecular Biology research laboratory and
pharmaceuticals industry in Bangladesh.
e:
asing95bio@gmail.com