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J Med Oncl Ther 2017 | Volume 2 Issue 4
Oncology and Biomarkers Summit
November 27-28, 2017 | Atlanta, USA
Annual Congress on
Evaluation of circulating endometrial cells as a biomarker for endometriosis
Chen Zhang
Peking University People’s Hospital, China
Introduction:
Endometriosis (EM) is a common disease
among women of reproductive age but significantly under
diagnosed in the absence of a reliable clinical marker. It has
been reported that circulating endometrial cells (CECs) were
present in peripheral blood of women with EM, providing
clear and specific evidence of the presence of ectopic
lesions. However, the clinical value of CECs is still unknown.
Methods:
In this study, we established a method with high
detection rate of CECs, examined the prevalence of CECs
in patients with ovarian EM and compared the diagnostic
performance with serum CA125, proposed a hypothesis of
the pathogenesis of EM from the new perspective of CECs.
Results:
The peripheral blood samples were collected
from 59 participants and the blood cells were isolated for
immunofluorescence staining via microfluidic chips. The cells
that were positive for vimentin/cytokeratin and estrogen/
progesterone receptor and negative for CD45 were identified
as CECs. The detection rate of CECs reached 89.5% (17/19) in
the EM group, which was significantly higher than that of the
control group (15% (6/40), P<0.001) and was independent of
menstrual cycle phases. Furthermore, a positive CEC assay
detected 4/5 cases of stage I-II EM. In contrast, a positive
CA125 test had limited value in detecting EM (13/19, 68.4%)
and only detected one case of stage I-II EM.
Conclusion:
In brief, CECs are a promising biomarker for EM
with great potential for non-invasive diagnostic assay.
Speaker Biography
Chen Zhang is working on Ph.D. in Gynecology and Obstetrics at Peking University
Health Science Center since September 2016. Her research interest has been on
endometriosis and ovarian cancer, mainly focusing on biomarkers research and
molecular mechanisms. Combining the characteristics of endometriosis and document
reports, she and her team members found an effective and efficient method to detect
and identify circulating endometrial cells in peripheral blood utilizing proprietary
microfluidic chip which reveals a novel and promising diagnostic approach for
endometriosis.
e:
zhangchen20080925@163.com