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alliedacademies.comYEARS
LASER, OPTICS AND PHOTONICS
World Conference on
Obesity Summit 2018 & Diabetes Conference 2018 & Laser Photonics Conference 2018
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
Mahbub Hasan et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C7-020
MOMORDICA CHARANTIA
INHIBITS
CARBOHYDRATE DIGESTION AND
ABSORPTION IN THE GI TRACT
Mahbub Hasan, Kazi Ishtiaq Ahmad, Limu Parvin, Kamol Krishna
Nandy
and
JMA Hannan
Independent University, Bangladesh
M
omordica charantia
fruits has been widely used as a traditional drug
throughout Asia. The goal of the present study is to explore the
mechanism of its anti-hyperglycemic activity related to the inhibition of
carbohydrate digestion and absorption in the gut. The dried-powder-fruits
of
M charantia
were extracted with methanol. Sucrose malabsorption was
assessed in 20 h-fasted rats by determining the portion of sucrose remaining
in six different parts of gastrointestinal tract after sucrose administration (2.5
g/kg b.w). Inhibition of glucose absorption in the gut was evaluated by utilizing
the in situ perfusion of small intestine. For investigation of disaccharides
activity, the extract was orally administered orally to 20 h-fasted rats. The
small intestines of the sacrificed rats were isolated and homogenized after 60
min. At 37 °C, 40 mmol sucrose mixed homogenate (l) was incubated for 60
min. Disaccharides activity was determined by glucose generated as a result
of sucrose digestion as mol-mg glucose/protein/h. After administration of
M
charantia
extract with the sucrose load was shown that the residual sucrose
quantity was increased significantly (p<0.01) in the gastrointestinal tract,
particularly in the upper intestine after 30 min, in the whole intestine and
cecum after 60 min and 120 min. At 240 min sucrose was not traceable in
the gut. During 30 min of perfusion the intestinal glucose absorption with
glucose was almost constant. When
M charantia
extract was administrated
with the glucose solution, the glucose absorption percentage was decreased
during whole perfusion period (p<0.05). Disaccharidase (sucrose) activity
was significantly (p<0.01) decreased in extract treated rats. In conclusion,
the anti-hyperglycemic action of
M charantia
fruit-fed-red are mediated partly
via: delayed intestinal carbohydrate digestion and absorption.
Mahbub Hasan has recently completed masters of
pharmacy, on clinical pharmacy and molecular phar-
macology from East West University and also com-
pleted bachelor of pharmacy from Stamford Universi-
ty, Bangladesh. His research work was done on active
supervision of JMA Hannan. He has published review
scientific articles in reputed journals on “Nanotechnol-
ogy drug delivery system: Tools in advances pharma-
ceutical & healthcare and contemporary investigation
on nasal spray drug delivery system”. Also has an
international Conference Paper on “Gastro retentive:
A novel drug delivery system” on 4-5 February 2017
Dhaka, Bangladesh.
mahbubhasan.cr7@gmail.comBIOGRAPHY