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N o v e m b e r 0 5 - 0 6 , 2 0 1 8 | P h i l a d e l p h i a , U S A
3rd INTERNATIONAL OBESITY SUMMIT AND EXPO
&
&
DIABETES, NUTRITION, METABOLISM & MEDICARE
2nd International Conference on
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
LASER, OPTICS AND PHOTONICS
World Conference on
Obesity Summit 2018 & Diabetes Conference 2018 & Laser Photonics Conference 2018 Biomedical Research | ISSN: 0976-1683| Volume 29
Afra Haque et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C7-020
ANTI-HYPERGLYCEMIC ACTIONS OF
NIGELLA SATIVA MEDIATED BY INHIBITION
OF CARBOHYDRATE DIGESTION AND
ABSORPTION IN THE GUT
Afra Haque, Abir Huzaifa, Afrina Sanju, Nur-A-Safa, Kazi Ishtiaq
Ahmad, Mahbub Hasan, Kamol Krishna Nandy and JMA Hannan
Independent University, Bangladesh
Previously Nigellasativaseedshavebeendocumentedasa traditional herbal
treatment for diabetes. Anti-hyperglycemic activity of its seed extract, in
the postprandial state, indicates that the extractmay interferewith the glucose
absorption in the gut. The present study aims to explore the mechanism of its
anti-hyperglycemic activity related to the inhibition of carbohydrate digestion
and absorption in the gut. The dried powdered seeds of N sativa were
extracted with methanol. Rats were fasted for 20 h prior to the experiment.
Sucrose (2.5g/Kg/5ml), average 443mg) with or without extract (0.5 g/kg)
was administered orally. Following administration rats were sacrificed after
30 min,60 min, 120 min respectively. Sucrose malabsorption was evaluated
in the rats by measuring the amount of sucrose remaining in six different
parts of gastrointestinal tract. With ice- cold saline (10 ml) each segment was
washed out, for acidifying H2SO4 (2 ml) was added and centrifuged at 3000
rpm for 10 min. To hydrolyze the sucrose the supernatant thus obtained was
boiled for 2 hours and then neutralized with NaOH (approximately 2.5 ml).
Glucose Oxidase (GOD-PAP) method was used to measure the amount of
glucose liberated from residual sucrose in the gastrointestinal tract. From the
amount of liberated glucose the sucrose content in the gastrointestinal tract
was calculated. When extract of N sativa was administered simultaneously
with the sucrose load, the residual sucrose content in the GI Tract was
increased significantly (p<0.01) and (p<0.05 respectively) in the stomach at
30min and 1h, especially in the upper intestine at 1h, in the middle intestine
at 1h, in the lower intestine at 30min and 2h (p<0.01), in small intestine, large
intestine and cecum at 30min and 1hr. The anti-hyperglycemic activities of N
sativa in Long Evans rat are related to retardation of intestinal carbohydrate
digestion and absorption in the gut.
Afra Haque completed her bachelor of pharmacy
from East West University (EWU), Dhaka, Bangladesh.
During her bachelors program she has done her the-
sis project on Pharmacology on the topic “Anti-hyper-
glycemic activity of Nigella sativa plant seed on Long
Evans rat” under the supervision of JMA Hannan. She
has been awarded dean’s list scholarship and full free
scholarship during her bachelors program.
BIOGRAPHY