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Journal of Neurology and Neurorehabilitation Research | Volume 3

August 23-24, 2018 | Paris, France

Neurology and Neurological Disorders

International Conference on

Role of mir-185 and SEPT5 Genes in Pathogenesis of Parkinson’s disease in animal model

Kambiz Hassanzadeh

and

Arman Rahimmi

Kurdistan University of Medical Sciences, Iran

Backgrounds and aim:

The pathophysiology of Parkinson’s

disease (PD) has been under immense investigations for

more than a century. However, there are still many unknown

mechanisms toward the disease. Clarifying the molecular

mechanisms involved in PD, can assist in developing novel

and efficient therapies. Recently, studies showed genes

located on the human chromosome location 22q11.2 might

be involved in development of PD. Therefore, our research

aimed to evaluate the role of two genes located on the

chromosomal location (mir-185 and SEPT5) which were

the most probable candidates based on our bibliography.

Methods:

Male wistar rats were divided in two groups,

randomly (n = 8). One group received rotenone injections

(1.5 mg/day s.c.) for 45 consecutive days. The other group

(control) only received rotenone injection vehicle (sunflower

oil). Behavioral tests including rotarod, rearing and bar tests

were performed at baseline and 45th day. Substantia nigra

and striatum were extracted from the animals’ brain. The

expression of mir-185 and SEPT5 genes were measured

at mRNA level using syber green real-time PCR technique.

Results:

the results of behavioral tests showed significant

decrease in performance of rotenone treated group compared

to control group (P< 0.05). The level of mir-185 and SEPT5

genes also decreased significantly in substantial nigra of

rotenone treated group compared to control group (P< 0.05).

However, there was no significant difference between two

groups in expression of mir-185 and SEPT5 genes (P< 0.05).

Conclusion:

the results of current study introduce mir-185 and

SEPT5genesasnovelgenesparticipatinginpathophysiologyofPD.