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Biol Med Case Rep 2017 | Volume 1 Issue 2
November 06-07, 2017 | New Orleans, USA
Nanomedicine & Healthcare
Global Meet on
Highly efficient gene carriers based on carbon nanotubes: A newmethod for friedel-crafts acylation of
single-walled carbon nanotube using trinuclear oxo-centered iron complex
Azadeh H Nia
Mashhad University of Medical Sciences, Iran
C
arbon nanotubes (CNTs) have been extensively explored
due to their unique physical, optical and chemical
properties. Their biocompatibility and ability to penetrate
cell membrane have extended their application to biomedical
research. Chemical functionalization is the main method to
overcome the low solubility of CNTs. Friedel-crafts acylation,
non-destructive method preserving the superior properties
of CNTs, is the least studied method compared to other
chemical functionalization approaches. We previously
reported a method for friedel-crafts acylation of multi-
walled carbon nanotube (MWNT). In this study, we report
a novel method using trinuclear oxo-centered iron complex
for acylation of single-walled carbon nanotube (SWNT) using
different alkyl carboxylic acidswithdifferent alkyl chain length
(C
6
, C
10
and C
16
). Functionalized SWNTs (F
6
, F
10
and F
16
) were
then conjugated with polyethylenimine (PEI) with different
molecular weights (MW: 1800, 10000 and 25000 kDa). In this
project, different nanocarriers were synthesized all capable
of effective condensation of plasmid DNA. All nanocarriers
were characterized by TGA, TEM, zetasizer, IR spectra.
Cytotoxicity of all vectors were evaluated by MTT assay. All
synthesized nanocarriers were tested for their transfection
efficacy after condensing plasmid encoding enhanced green
fluorescent protein (EGFP). The effect of length of alkyl chain
and molecular weight of PEI on transfection efficiency of
final conjugates were studied. Results of luciferase assay and
fluorescence live cell imaging confirmed the high levels of
EGFP gene expression.
Speaker Biography
Azadeh H Nia has completed her PhD in the field of Organic Chemistry at Ferdowsi
University and Postdoctoral studies in nanomedicine at Mashhad University of Medical
Sciences. Her area of research covers biomedical application of nanomaterials in
health sciences. She has recently joined World Academy of Medical Sciences (WAMS)
as a Faculty Member and Editor In-Chief of
WAMS journal.
She has served for more
than 10 years in academic research positions, she has published more than 12 papers
in reputed journals and has been serving as an Editorial Board Member of different
journals in the nanotechnology field.
e:
hashemnia.az@gmail.com