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Biol Med Case Rep 2017 | Volume 1 Issue 2
November 06-07, 2017 | New Orleans, USA
Nanomedicine & Healthcare
Global Meet on
Investigating the effects of electrical stimulation via gold nanoparticles on
in vitro
neurite outgrowth:
Perspective to nerve regeneration
Moein Adel
Tehran University of Medical Sciences, Iran
Statement of the problem:
Following the injury of nervous
tissue spinal cord injuries, axons do not regenerate
appreciably in their native environment and current clinical
approach to treating damaged nerves is inefficient; thus,
medical treatment approaches are needed. Neural tissue
engineering research field has been progressed by using
different approaches especially for repairing of damaged
neural cells. In addition, it is known that electrical stimulation
can be used for neurite growth and nerve regeneration.
Methodology & theoretical orientation:
In this study,
conductive properties of gold nanoparticles (GNPs,
39 nm) and their contribution to the enhancement of
electrical stimulation to nerve cells have been conducted.
In experimental section, polyethyleneimine (PEI) polymer
coated cover glasses was used to create a positively charged
glass surface and adsorption of GNPs was used in conjugation
with this polymer coated substrate. Subsequently, PC12
cells were cultured on the modified glass surface and
pulsed electric field of 1.5 V, 20 Hz was applied as electrical
stimulation for 55 min duration.
Findings:
Images from FESEM showed a uniform distribution
of GNPs on glasses surface. In addition, enhanced neurite
outgrowth (120 μm) using electrical stimulation was
determined by inverted phase contrast microscopy images.
Conclusion & significance:
Finally, our study showed that
pulsed current stimulation induced neurite outgrowth
of PC12 cells adhered to the GNPs coated surfaces.
Altogether, synergist combination of GNPs together with
pulsed electrical stimulation can be used for enhanced
nerve regeneration. Our future works will direct towards
optimizing properties of NPs and stimulation parameters for
in vivo
nerve regeneration and do a comparative study with
other nanomaterial including silk, carbon materials etc.
Speaker Biography
Moein Adel has his expertise in nerve regeneration and nanotechnology. His open and
contextual evaluation model creates new combination and optimization pathways
for treatment of CNS damages. He has built this model after years of experience in
research, evaluation and teaching both in research and education institutions. The
foundation is based on fourth generation evaluation (Guba and Lincoln, 1989) which
is a methodology that utilizes the previous generations of evaluation: measurement,
description and judgment. It allows for value-pluralism. This approach is responsive to
all stakeholders and has a different way of focusing.
e:
m-adel@razi.tums.ac.ir