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allied
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May 16-17, 2019 | Prague, Czech Republic
2
nd
International Conference on
22
nd
International Conference on
Nanomaterials and Nanotechnology
Advanced Nanoscience and Nanotechnology
Joint Event
&
Journal of Materials Science and Nanotechnology | Volume 3
Mater Sci Nanotechnol, Volume 3
Design and synthesis of nanoparticles based chitosan for improving drug delivery
across blood-brain barrier
Martin P
1
, Savin C
2
, Peptu C A
2
, Popa M
2,3
, Sevin E
1
, Gosselet F
1
, Chaveriat L
1
1
Université d'Artois, France
2
University of Iasi, Romania
3
Academy of Romanian Scientists, Romania
W
e prepared nanoparticles based on chitosan grafted
with poly (ethylene glycol) (PEG) methacrylate. These
nanoparticles were then loaded with doxorubicin. The
nanoparticles have been fully characterized: laser diffraction,
electron microscopy, kinetic of swelling of nanoparticles. The
size distribution curve of the nanoparticles attests that the
averagediameter is450nm.Theanalysisofthemorphologyof
the nanoparticles proved the obtaining of spherical particles,
well individualized. The process of loading and releasing
the drug into the particle is based on diffusion through the
hydrogel matrix. As a result, it depends on the swelling rate
of the particles in aqueous solutions. The pH of the solution
is also an important factor influencing the swelling degree.
The kinetics of the particle swelling process was studied in an
aqueous medium at acid pH (3.3) and weakly basic (7.4). The
swelling degree in both media is high enough, so the particles
may fall into the category of superabsorbent gels. The effect
is due to the high hydrodynamic volume of macromolecules
that are branched by PEG on chitosan chains. To determine
if these nanoparticles could be used as vehicle molecules
to treat neurodegenerative diseases, we then investigated
their toxicity using an
in vitro
model of the human blood-
brain barrier (BBB). Free doxorubicin nanoparticles did not
modify BLECs (Brain Like Endothelial Cells) permeability
meaning that these molecules do not show any toxic effects
at the BBB level. These nanoparticles could therefore be used
after functionalization with anti-transferrin antibodies to
cross the BBB and thus deliver doxorubicin in brain tumors.
e
:
patrick.martin@univ-artois.fr