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allied
academies
November 22-23, 2018 | Paris, France
Journal of Materials Science and Nanotechnology | Volume: 2
Materials Physics and Materials Science
International Conference on
Exploitation of
Enterobacter spp.
inmicrobial degradation of acrylamide: an environmental bioremedial
approach
Maulin P Shah
Enviro Technology Limited, India
A
widespread use of acrylamide, probably a neurotoxicant
and carcinogen, in various industrial processes has
led to environmental contamination. Fortunately, some
microorganisms are able to derive energy from acrylamide. In
the present work, we reported the isolation and characterization
of a novel acrylamide-degrading bacterium from domestic
wastewater in Chonburi, Thailand. The strain grew well in the
presence of acrylamide as 0.5% (W/V), at pH 6.0 to 9.0 and
25°C. Identification based on biochemical characteristics and
16S rRNA gene sequence identified the strain as
Enterobacter
spp.
Degradation of acrylamide to acrylic acid started in the
late logarithmic growth phase as a biomass-dependent pattern.
Specificity of cell-free supernatant towards amides completely
degraded butyramide and urea and 86% of lactamide. Moderate
degradation took place in other amides with that by formamide
> benzamide >acetamide > cyanoacetamide > propionamide.
No degradation was detected in the reactions of N,N-methylene
bisacrylamide, sodiumazide, thioacetamide, and iodoacetamide.
These results highlighted the potential of this bacterium in the
cleanup of acrylamide/amide in the environment.
e:
shahmp@uniphos.com