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Journal of Materials Science and Nanotechnology | Volume 3
October 07-08, 2019 | Frankfurt, Germany
Materials Science and Engineering
3
rd
International Conference on
Mater Sci Nanotechnol, Volume 3
Effect of feed supplementation with biosynthesized silver nanoparticles using leaf
extract of
Morus indica
L. V1 on
Bombyx mori
L.
Amit Kumar Mandal
Raiganj University, India
H
erein, we report the synthesis of silver nanoparticles
(AgNPs) by a green route using the aqueous leaf extract
of
Morus indica
L. V1. The synthesized AgNPs exhibited
maximum UV-Vis absorbance at 460 nm due to surface
plasmon resonance. The average diameter (~54 nm) of
AgNPs was measured from HR-TEM analysis. EDX spectra
also supported the formation of AgNPs, and negative zeta
potential value (-14 mV) suggested its stability. Moreover,
a shift in the carbonyl stretching (from 1639 cm-1 to 1630
cm-1) was noted in the FT-IR spectra of leaf extract after
AgNPs synthesis which confirm the role of natural products
present in leaves for the conversion of silver ions to AgNPs.
The four bright circular rings (111), (200), (220) and (311)
observed in the selected area electron diffraction pattern
are the characteristic reflections of face centered cubic
crystalline silver. LC-MS/MS study revealed the presence
of phytochemicals in the leaf extract which are responsible
for the reduction of silver ions. MTT assay was performed
to investigate the cytotoxicity of AgNPs against two human
cell lines, namely HepG2 and WRL-68. The antibacterial
study revealed that MIC value of the synthesized AgNPs was
80 µg/ml against
Escherichia coli
K12 and
Staphylococcus
aureus
(MTCC 96). Finally, the synthesized AgNPs at 10 µg/
ml dosages showed beneficial effects on the survivability,
body weights of the Bombyx mori L. larvae, pupae,
cocoons and shells weights via enhancing the feed efficacy.
e
:
amitmandal08@gmail.com