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Page 55

Journal of Materials Science and Nanotechnology | Volume 3

October 07-08, 2019 | Frankfurt, Germany

Materials Science and Engineering

International Conference on

Mater Sci Nanotechnol, Volume 3

Formative biofabrication using levitational bioassembly in high magnetic field

Vladimir Mironov

3D Bioprintig Solutions, Russia

everal research groups recently demonstrated the

principal feasibility of magnetic levitational bio-assembly

of tissue engineered constructs from living tissue spheroids

in the presence of paramagnetic ions. However, Gd3+ is

relatively toxic at concentrations above 50mMused normally

to enable magnetic levitation with NdFeB permanent

magnets. Using high magnetic field at HFML in Nijmegen, the

Netherlands, it was possible to performmagnetic levitational

assembly of tissue constructs from living spheroids prepared

from SW1353 osteosarcoma cell line with 100 times lower

concentration of Gd3+. The assembly of tissue constructs was

performed in a 50mm-bore, 30 Tesla Bitter magnet, equipped

with mounting for cuvettes filled with culture medium

containing spheroids and non-toxic concentrations of Gd3+.

The levitation conditions were initially adjusted using 170

µm polystyrene beads. To predict the theoretical possibility

of assembly, a zone of stable levitation in the horizontal and

vertical area of cross sections was previously calculated.

Round-shaped structures from the polystyrene beads and

tissue spheroids were assembled at 0.5 and 0.8 mM Gd3+ in

22T and 19T magnetic fields, respectively. The construct from

tissue spheroids partially fused after 3 hours of levitation.

The analysis of viability after prolonged exposure of strong

magnetic field showed the absence of significant cytotoxicity

or morphology changes in the tissue spheroids. High

magnetic fields works as a temporal and removal support or

so-called “scaffield”. Thus, formative bio-fabrication of tissue

engineered constructs from tissue spheroids in highmagnetic

field is a promising research direction.