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Journal of Systems Biology & Proteome Research

Volume 2

J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d

MASS SPECTROMETRY

AND PROTEOMICS

International Conference on

Md Matiur Rahman et al., J Syst Biol Proteome Res 2018, Volume 2

ANALYTICAL CHARACTERISTICS OF

NANO-ELECTROSPRAY OPERATED

UNDER SUPER-ATMOSPHERIC

PRESSURE

Md Matiur Rahman

and

Lee Chuin Chen

Beijing Institute of Technology, China

University of Yamanashi, Japan

igh-pressure nanoelectrospray ionization (nanoESI) source is a recently

developed technique in which the electrospray ionization is generated

inside an enclosed chamber with gas pressure higher than the atmospheric

pressure. In this paper, the performance of nanoESI under different gas

pressures, emitter position, ion inlet temperature, additive for desalination

are presented. Under a pressure of 2 bars, the nanoESI is almost eased from

the electrical discharge problem, and that offers a wider tuning window for

the emitter potential to produces a higher and more stable ion signal. With

optimized ion inlet temperature, the high-pressure operation facilitates the

generation of ion species of higher charge-state from the highly aqueous

solution, and produced less sodium adducts. A preparation method for the

high-throughput analysis of raw biological samples using disposable plastic

nanoESI emitter could be described.

High pressure nESI ion source