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Journal of Systems Biology & Proteome Research
|
Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
MASS SPECTROMETRY AND PROTEOMICS APPLICATIONS
Anil Batta
Govt. Medical College Amritsar, India
M
ass spectrometry (MS) is widely recognized as a powerful analytical tool for molecular research. MS is used by researchers
around the globe to identify, quantify, and characterize biomolecules like proteins fromany number of biological conditions or
sample types. As instrumentation has advanced, and with the coupling of liquid chromatography (LC) for high‐throughput LC‐MS/
MS, a proteomics experiment measuring hundreds to thousands of proteins/protein groups is now commonplace. While expert
practitioners who best understand the operation of LC‐MS systems tend to have strong backgrounds in physics and engineering,
consumers of proteomics data and technology are not exposed to the physio‐chemical principles underlying the information they
seek. Since articles and reviews tend not to focus on bridging this divide, our goal here is to span this gap and translate MS ion
physics into language intuitive to the general reader active in basic or applied biomedical research. Here, we visually describe
what happens to ions as they enter and move around inside a mass spectrometer. We describe basic MS principles, including
electric current, ion optics, ion traps, quadrupole mass filters, and Orbitrap FT analyzers.
akbattafarid@yahoo.co.inJ Syst Biol Proteome Res 2018, Volume 2