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Journal of Systems Biology & Proteome Research
|
Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
Gye Cheol Kwon, J Syst Biol Proteome Res 2018, Volume 2
CURRENT STATUS OF TRIGLYCERIDE
STANDARDIZATION IN KOREA, AN
EXPERIENCE FROM NATIONAL LIPID
STANDARDIZATION PROGRAM FROM
2016 TO 2017
Gye Cheol Kwon
Chungnam National University Hospital, South Korea
A
ccurate and precise measurements of triglycerides (TG) are crucial in
cardiovascular disease risk management. We examined standardized
data to clarify the performance of routine TG methods in Korea through
National Lipid Standardization Program from 2016 to 2017. Three samples
were sent to the laboratory for each cycle, twice a year. The participating
laboratories were required to repeat the sample measurements three times
a day for two days. Total glycerides were measured in Korean Centers for
Disease Control (CDC) by isotope dilution mass spectrometry, a method
designed by CDC in the United States. The target values of triglyceride
were determined by subtracting the free glycerol value measured by ReCCS
(Reference Material Institute for Clinical Chemistry Standards) in Japan. We
calculated the total coefficient of variance (CV), the percent bias, and the total
error for each sample. Two routine methods were largely used by laboratories;
The one is enzymatic method without glycerol blanking (nonblanking group)
and the other is the with glycerol blanking (blanking group). The mean
values of blanking group were consistently lower than those of nonblanking
group. Regardless of the method of measurement, the imprecisions of most
laboratories showed less than 2%, which qualified to the NCEP (National
Cholesterol Education Program) criteria. However, in relation to bias, in about
1/4 of the laboratories, the bias was found to exceed the 5% NCEP criteria
and appeared to be more prominent in the group using specific reagents.
There was no significant difference between the two groups in terms of
accuracy, but negative bias was more pronounced in nonblanking group than
in blanking group. Because of outperformed precision, few laboratories had
total error greater than 15%. In conclusion, the precision of triglycerides was
found to be satisfactory in most laboratories in Korea but the accuracy still
needs improvements.
Gye Cheol Kwon has completed his PhD in 1993
from Chungnam National University, South
Korea. Currently he is the director/professor
of Chugnam National University Hospital, vice
president of Korean External Quality Assess-
ment Scheme and will be the chairman of the
Korean Society for Laboratory Medicine from
2019. His research interest is in mass spec-
trometry, triglyceride and pre-analytical issues
in the laboratory.
kckwon@cnu.ac.krBIOGRAPHY