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Journal of Systems Biology & Proteome Research
|
Volume 2
J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d
MASS SPECTROMETRY
AND PROTEOMICS
International Conference on
APPLICATION OF TWO DIMENSIONAL HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY–MASS SPECTROMETRY ON
TESTING VITAMIN D
Deming Song
Food and Drug Administration Northeast Region Laboratory, USA
T
he use of a particle beam interface (PBI) in LC–MS has faded because of its poor resolution and low sensitivity for many
compounds. It also does not performwell in reversed-phase systemswith a high percentage of water. Thismanuscript describes
two-dimensional LC to overcome the problems of PBI, both in the identification of unknown samples and in the quantitative
determination of metabolites of vitamin D in human plasma. For the identification of unknown components, the reversed-phase
solvent in the first dimension was transferred to an isocratic normal solvent system in second dimension by column switching.
Moreover, only the peaks of interest (components) in the first dimension were transferred into the second LC column for mass
analysis. For the quantitative determination of the metabolite of vitamin D, the peak width of analyte in the second dimension was
greatly narrowed, and interference was excluded such that high sensitivity and resolution resulted. The limit of quantification for
the test metabolite of vitamin D in human plasma can reach 0.050 ng/ml with injection volume of 50 ml. A new instrumentation,
Absiex LC-MS-MS is going to be employed in the new study of Vitamin D test.
deming.song@fda.hhs.govJ Syst Biol Proteome Res 2018, Volume 2