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Journal of Systems Biology & Proteome Research

Volume 2

J u n e 2 5 - 2 7 , 2 0 1 8 | D u b l i n , I r e l a n d

MASS SPECTROMETRY

AND PROTEOMICS

International Conference on

DIAGNOSTIC ACCURACY OF CANNABINOID TESTING BY LIQUID

CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY IN HUMAN HAIR

Alveena Younas, Asif Nawaz, Ayesha Hafeez, Aamir Ijaz, Naveed Asif

and

Abdul Manan

Armed Forces Institute of Pathology (AFIP) Rawalpindi, Pakistan

Objective:

To determine the diagnostic accuracy of Cannabinoids testing by Liquid Chromatography-Tandem Mass Spectrometry

(LC-MS) in human hair and to compare it with urine for detection of cannabis use in civil heavy vehicle drivers

Materials and Methods:

This diagnostic accuracy(validation) study was carried out at Department of Forensic Medical Sciences

Laboratory (FMSL), Forensic Toxicology Section, Armed Forces Institute of Pathology Rawalpindi, Pakistan. Hair and urine samples

of about 151 civil heavy vehicle drivers were collected from various urban and rural areas of Punjab.

Result:

Sampling technique was non-probability convenient. About 10 ml of urine volume was collected and stored at -20 °C. Hair

strands, about the thickness of a pencil shaft, were collected from the posterior vertex of

was cut as close to the root as

possible, and kept at room temperature till further analysis Separation of compounds was done on Agilent Poroshell 120 EC-C18

Al the 151-male civil heavy vehicle drivers, who were included in the study, were categorized into three main groups. There were

69(71.5%) truck drivers,43(28.5 %) were 20-wheeler drivers, whereas bus drivers were 39(25. 8%).Mean age was 36±10.82 years.

Subjects were stratified according to the age into four main groups: 20-25 y: 28(18.5%); 26-40 y: 73(48.3%), 41-60 y:47(31.1%)

and >60 y:3(2%).

Conclusion

: This study highlights the importance of hair as an alternative biological matrix due to its good diagnostic yield, easy

non -invasive specimen collection and distinctive potential of analyte stability, as well as wider period of detection as compared

to urine.

J Syst Biol Proteome Res 2018, Volume 2