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Virology Research Journal
|
Volume 2
Page 27
allied
academies
IMMUNOLOGY AND CELL BIOLOGY
BACTERIOLOGY AND INFECTIOUS DISEASES
&
Global Summit on
Global Congress on
J u n e 2 5 - 2 6 , 2 0 1 8 | A m s t e r d a m , N e t h e r l a n d s
Joint Event on
DETECTION AND SUSCEPTIBILITY
PATTERN OF BIOFILM-PRODUCING
PSEUDOMONAS AERUGINOSA AND
USING CRISPR/CAS SYSTEMS TO
KNOCK-OUT BIOFILM-SPECIFIC
ANTIBIOTIC RESISTANCE GENES
Muhammad Sadeqi Nezhad
Islamic Azad University, Iran
Backgrounds:
Biofilm plays an important role in chronic diseases and their
eradication is very challengeable, when bacteria confront with antibiotics
or strong immune system response they have the choice whether to be
planktonic cells or form a biofilm, producing extra cell materials to enhance
their survival. The aim of this study was the assessment of incidence and
antibiotic algorithms of biofilm-producing
Pseudomonas aeruginosa
, an
opportunistic pathogen and one of the most frequent causes of infectious
disease in vulnerable patients.
Methods:
A total of 100
P.aeruginosa
isolates were collected from five
different clinical specimens and wards of the fifth Azar Hospital, Gorgan, Iran
during November 2017. However, after isolating of samples under sterilized
conditions, these strains have been identified as a
P.aeruginosa
through
appropriate biochemical procedures and their antibiotic patterns according
to NCCLS disk methodology have been examined; afterward, ELISA method
was employed for the detection of biofilm producing
P.aeruginosa
.
Results:
Out of 100 clinical isolated
P.aeruginosa
31(31%) of themwere biofilm
producer. The frequency of biofilm positive strains among specimens have
been observed; 56.2% from burned wounds, 36.4% from urines, 22.2% from
respiratory secretions, 19.4% from blood cultures and 16.7% of the strains
were biofilm positive from normal wound cultures (P=0129). Besides, 50%
of biofilm-producing
P.aeruginosa
were isolated in internal section followed
by burned section (45.8%), ICU section (29.4%), surgical section (15.8%) and
9.2% in pediatric neurology section (P=0129). Furthermore, biofilm-producing
P.aeruginosa
indicated impressive resistance patterns to piperacillin (49.2%),
Imipenem (49.2%), ciprofloxacin (47.6%), gentamicin (46.7%), ticarcillin
(44.1%), cefepime (38.9%), ceftazidime (34.9%), ceftriaxone (34.3%), co-
trimoxazole (34.1%) and cefotaxime (31.6%) respectively.
Conclusions:
This study demonstrated that there is a discrepancy in the
outbreak of biofilm-producing
P.aeruginosa
among various specimens and
also the pattern of antibiotic susceptibility and resistance did not follow a
specific algorithm.
Muhammad Sadeqi Nezhad is majoring in Clinical
Laboratory Science (BSc), Gorgan Islamic Azad
University. He is a passionate, research-driven stu-
dent looking to possess diversity of knowledge and
necessary skills at Oncology/Pathology in medical
school to begin a career in clinical research to dis-
cover diagnostic methods and treatment.
Labmsn9@gmail.comBIOGRAPHY
Muhammad Sadeqi Nezhad, Virol Res J 2018, Volume 2