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Virology Research Journal
|
Volume 2
Page 25
allied
academies
IMMUNOLOGY AND CELL BIOLOGY
BACTERIOLOGY AND INFECTIOUS DISEASES
&
Global Summit on
Global Congress on
J u n e 2 5 - 2 6 , 2 0 1 8 | A m s t e r d a m , N e t h e r l a n d s
Joint Event on
ANALYSIS OF THE ROLE OF GENETIC
POLYMORPHISMS OF INNATE
IMMUNE SIGNALING FACTORS IN
INFLAMMATORY DISEASE
Gurumoorthy Kaarthikeyan
Saveetha Dental College, India
P
eriodontitis is a chronic inflammatory disease of multifactorial etiology.
The gram negative anaerobes are the main etiological agents in
causing periodontal destruction. The genetic risk factors plays a major
role in determining the susceptibility to periodontal disease. The virulence
factors of these anaerobes like lipopolysacharide (LPS) are screened by
the pattern recognition receptors like Toll like receptors and innate immune
signaling cascade is activated. This signaling cascade is regulated by many
microRNAs like
miR146a
. This
microRNA146a
negatively regulates TLR4
pathway by blocking interleukin 1 receptor associated kinase (IRAK1),
TNFreceptor associated factor (TRAF6). This
miR146a
is in turn regulated
by apolipoprotein E(apoE). ApoE is a major cholesterol carrier and plays
an important role in maintaining lipid homeostasis. ApoE selectively
regulates TLR4- and TLR3-mediated signaling. The apoE may suppress
the Th1 immune response by modulating IL-12 production. The inactive
pro inflammatory cytokine IL-1beta secreted by this signaling cascade
is activated by Nod like receptors called
NLRP3
in cytoplasm. The genetic
changes of these signaling and regulatory factors of innate immune system
might determine the susceptibility to periodontal destruction. Thus the aim
of this study was to determine the association of the genetic polymorphisms
of
miR146a
, apoE and
NLRP3
with periodontitis in south Indian population.
The study was approved by the institutional ethics committee of Saveetha
university (017/10/2013/IEC/SU). The study included three groups- chronic
periodontitis group (n=81), aggressive periodontitis group (n=80) and healthy
controls (n=167). After getting informed consent, five ml of venous blood was
collected by veinpuncture. DNA extraction was done according to modified
Millers et al technique. The gene polymorphisms of
miR146a
(rs2910164),
NLRP3
(rs10802501, rs10754558), apoE was analyzed using specific primers
in real time PCR.
Conclusion:
Thus our study concludes that the allelic frequency of
NLRP3
(rs
10802501),
miR146a
(rs 2910164) and apoE polymorphisms were associated
with periodontitis in south Indian population. The biological plausibility of this
association has to be analysed with further studies.
Gurumoorthy Kaarthikeyan is working at Saveetha
Dental College since 2007. He is currently holding
the designation as Professor and Clinic Head (UG)
of the Department of Periodontics. He is the Co-or-
dinator for implant approval committee and he is
the Member of Scientific Review Board –Saveetha
University. He has 37 publications in various inter-
national and indexed journals. He has delivered
guest lectures at various national and international
conferences. He is a Reviewer and Editorial Board
Member in various journals.
drkarthik79@yahoo.co.inBIOGRAPHY
Gurumoorthy Kaarthikeyan, Virol Res J 2018, Volume 2