4 / 11
Page 36
allied
academies
Curr Trend Cardiol. 2017 | Volume 1 Issue 3
September 18-19, 2017 | Toronto, Canada
Annual Conference on
HEART DISEASES
Background:
Atherosclerosis is a chronic inflammatory
disease involving immunological activity, inflammatory
cells, proinflammatory cytokines, and primitive proteins.
Natural food ingredients are highly consumed to prevent
atherosclerosis. People consuming purple sweet potato
(
Ipomoea batatas
) have lower incidence of coronary heart
disease.
Objective:
The objective of this study is to prove the
mechanism of atherosclerosis prevention through the
provision of purple sweet potato (
Ipomoea batatas
).
Methods:
This research used a randomized post-test only
control group design using 18 white rats (
Rattus norvegicus
wistar strain
) that were divided into three groups containing
six rats in each group, namely, the first group was normal
group, the second group was given atherosclerotic diet,
and the third group was a treatment group given diet of
atherosclerotic and purple sweet potato (
Ipomoea batatas
)
extract orally (26.6 mg). After 90 days of treatment,
blood sampling was carried out for measuring the levels
of extracellular Heat Shock Protein 70 (e HSP70), high
sensitivity c-reactive protein (hs-CRP), lipoprotein associated
phospholipase A2 (Lp-PLA2) enzyme using ELISA and
measuring the intracellular Heat Shock Protein 70 (i HSP70)
expression in the monocytes using immunocytochemistry
and histopathologic observation on the number of foam
cells in rats’ aorta.
Results:
The administration of purple sweet potato
(
Ipomoea batatas
) extract is proved to decrease hs-CRP
level and the number of foam cells in the aorta. Increase
of i HSP70 expression in monocytes, activity of Lp-PLA2
enzyme and e HSP70 level in circulation occur. Path analysis
showed two significant paths in atherosclerosis prevention:
Ipomoea batatas - i HSP70-foam cell and Ipomoea batatas
- i HSP70 - e HSP70 - Lp-PLA2-foam cell. Path of
Ipomoea
batatas
- i HSP70-foam cell is more dominant to prevent
atherosclerosis. The role of intracellular Heat Shock Protein
70 (i HSP70) is dominant on the path as molecular cheperon
fuctioned as cytoprotective on monocyte.
Conclusion:
The extract of purple sweet potato (
Ipomoea
batatas
) can reduce hs-CRP, foam cell and improve i HSP70,
Lp-PLA2 and e HSP70. The main line in the prevention of
atherosclerosis is through Ipomoea batatas - i HSP70-foam
cell and is dominated by the role of i HSP 70.
Speaker Biography
Meddy Setiawan, MD, PhD is an Internal Medicine Specialist in Malang, Indonesia.
He received his medical degree and completed his medical residency at Brawijaya
University. He received his PhD degree in medicine from University of Airlangga.
Currenty he is affiliated with University of Muhammadiyah Malan and University of
Muhammadiyah Malang Hospital as lecturer and internal medicine specialist. As an
Internist and a lecturer, he has interests in conducting a research on Atherosclerotis.
Besides, he has also developed a textbook on Endocrine and the one on
Cardiocerebrovascular for his students. He has published his researches on the field of
Atherosclerotis such as 1) The Effect of Extract from Pericarp of Mangosteen (Garcinia
mangostana linn) as an Antioxidant in Rats Models of Atherosclerotis ( published in
Journal of Cardiology Indonesia, 2012), and 2) The Effect of Virgin Coconut Oil (VCO)
on the Regression of Foam Cell and the Ratio Decrease of LDL/HDL Cholesterol of
White Male Rat Atherosclerotic (Rattus novergicus strain wistar) published in Journal
of Sain Med, 2009. He also has already owned a patent for his invention on Seaweed as
Antihipertensive Therapy : The Formulation of Seaweed Effervescent Tablet (Eucheuma
spinosum) as an angiotensin I converting enzym (ACE) a natural inhibitor in 2015.
As a lecturer and a clinician, he also actively involves in some seminars, simposium
and workshops, either as a participant or a presenter. In May, 2017 he presented
his research on Basic Molecular Biology Course XV as a keynote speaker, entitled :
The Role of Primitive Protein Heat Shock Protein (HSP) on chronicle inflammation
(Atherosclerosis). Besides, he also has received some awards firstly, from The Ministry
of Culture and Education Republic of Indonesia as an advisor of Students Creativity
and Research in 2013, secondly, from The Ministry of Health Republic of Indonesia as
a medical practitioner for Indonesian Hajj in Saudi Arabia in 2012, 2014, thirdly from
Haramain Award from the Department of Religion and Health Republic of Indonesia as
a Researcher of Hajj Health in Saudi Arabia in 2014. Next, an award as an exemplary
lecturer in the Faculty of Medicine in Muhammadiyah University in Malang in 2017
and finally, an award from the President of Indonesia Satyalencana Karya Satya X Years
in 2016.
e:
meddy_umm@yahoo.comAtherosclerosis prevention mechanism through purple sweet potato (
Ipomoea batatas
) consumption:
Roles of intracellular HSP 70, extracellular HSP 70, Lipoprotein associated phospholipase A2 (Lp-PLA2),
high sensitivity C-reactive protein (hs-CRP) toward foam cell
Meddy Setiawan
1
, Djanggan Sargowo
2
and
Suhartono Taat Putra
3
1
University of Muhammadiyah Malang, Indonesia
2
Dr. Saiful Anwar General Hospital, Indonesia
3
Airlangga University, Indonesia