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Curr Trend Cardiol. 2017 | Volume 1 Issue 3
September 18-19, 2017 | Toronto, Canada
Annual Conference on
HEART DISEASES
Objective:
The aim of this study was determination of the
effect of Anethumgraveolens extract and anethumgraveolens
(dill) tablet on lipid profile, liver enzymes and histological
change and gene expression and enzymatic activity of LDL-R
and HMG-CoA reductase in control and high cholesterol fed
hamsters(HCD).
Materials & Methods:
Golden Syrian male hamsters (130
± 10 g) were randomly divided into 6 groups (n=6) and
received daily as following: Group 1: chow +2% cholesterol
+ 0.5% cholic acid, group 2: chow + 100 mg/kg hydroalcholic
extract of dill +2% cholesterol + 0.5% cholic acid, group
3: chow + 200 mg/kg hydroalcholic extract of dill +2%
cholesterol + 0.5% cholic acid, group 4: chow + 100 mg/
kg dill tablet + 2% cholesterol+0.5% cholic acid, group 5:
chow + 200 mg/kg dill tablet +2% cholesterol+0.5% cholic
acid, and group 6: chow. After 1 month feeding, animals
were anesthetized and sacrificed, biochemical factors were
determined enzymatically. LDL-R and HMG-CoA reductase
mRNA level was measured by Real time PCR and its activity
was determined spectrophotometrically.
Results:
Compared with hypercholesterolemic group-1,
lipid profile, blood glucose and liver enzymes significantly
decreased in all dill tablet-received or dill extract-treated
groups (p<0.05). The changes in HMG-CoA reductase gene
expression level and enzyme activity were not significant
in animals received 100 mg/kg of hydroalcholic extract or
dill tablet, but werereduced in animals received 200 mg/
kg of extract or tablet. The expression of LDL-R significantly
increased in animals received 200 mg/kg of extract or
tablet. Liver and heart antioxidant significantly increased by
Anethum graveolens. Liver histopatological changes were
normalized by Anethum graveolens.
Conclusion:
Dill can significantly reduce HMG-CoA reductase
activity and its gene expression level in hypercholesterolemia.
Anethum graveolens can also significantly increase LDL-R
gene expression in HCD animals. This study showed that dill
extract and dill tablet has potential hypocholesterolaemic
properties in hamsters by inhibition of HMG-CoA reductase
activity.
e:
7abbasi@gmail.comLDL-R, HMG-CoA reductase genes expression, antioxidant capacity and histological changes induced
by Anethum graveolens in hypercholesterolemic hamsters
Ebrahim Abbasi Oshaghi
1
, Iraj Khodadadi
1
, Fatemeh Mirzaei
2
, Heidar Tavilani
1
and
Mohamad Taghi Goodarzi
1
1
Hamadan University of Medical Sciences, Iran
2
Kermanshah University of Medical sciences, Iran