genetic-engineering-2019-scientifictracks

10 / 10

allied
academies
Page 29
Notes:
November 07-08, 2019 | Melbourne, Australia
Molecular Biology and Genetic Engineering
International Conference on
Journal of Research and Reports on Genetics | Volume 3
Signature pattern of gene expression and signaling pathway in premature diabetic patients
uncover their correlation to early age coronary heart disease
Salma Ahmadloo1, Ling King Hwa2, Ahmad Fazli3 and Patimah Ismail1
1 Shahid Beheshti University, Iran
2 Harvard Medical School, USA
3 Serdang Hospital, Malaysia
Coronary Heart Disease (CHD) is still the number-one killer
in the world. The number of people with premature CHD
has more than tripled in the past 40 years and the figures are
still growing. Notably, many of the patients with CHD have
diabetes mellitus (DM). This study was carried out for the
purpose of profiling expression of DM associated genes and
identify related biological process and modulated signaling
pathways of Malaysian male subjects with CHD from three
ethnic groups, namely Malay, Chinese and Indian. In order to
achieve the goal, four groups of subjects were divided into:
1) healthy subjects; 2) subjects with only DM; 3) subjects
with only CHD, and 4) subjects with CHD + DM. The RNA was
extracted from blood specimens by mean of commercial
extraction kits. The RT2 Profiler™ PCR Array was utilized to
determine gene profiling on group 1 and group 2, group 1 and
group 3, group 1 and group 4. To validate the results of RT2
profiler™ PCR Array, significantly dysregulated genes were
selected and validation was conducted through Q-RT-PCR in
a larger and independent population. For this purpose, new
subjects were divided into 1) healthy subjects. 2) Subjects
with DM+CHD. 12 significantly dysregulated genes related
to diabetes and Toll-Like receptor signaling pathway were
identified which may be a culprit to susceptible diabetic
patients to CHD development. In Silico experiments imply a
role for inflammatory responses in the circulating leukocytes
as a biomarker reflecting initiation of CHD in patients with
DM. In conclusion, some differentially dysregulated genes
and modulated pathways were identified which warrant
further investigation in the setting of CHD and its risk
factors. It is hoped that a greater understanding of genetic
predisposition to CHD will unravel clues to its etiology and
allow development of novel diagnostic and therapeutic tools
to permit targeted interventions to reduce this global health
burden.
Speaker Biography
Salma Ahmadloo has completed her Ph.D. in the field of Medical Genetics
from Universiti Putra Malaysia. She is a Postdoc fellow in Shahid Beheshti
University of Iran. She is an experienced Senior Researcher with a
demonstrated history of working in the higher education industry.
e:
Salma Ahmadloo et al., J Res Rep Genet 2019, Volume 3
DOI: 10.35841/2591-7986-C1-002