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Journal of Gastronenterology and Digestive Diseases
|
Volume 3
J u n e 2 5 - 2 6 , 2 0 1 8 | D u b l i n , I r e l a n d
GASTROENTEROLOGY
International Conference on
CHARACTERIZATION OF
ESCHERICHIA COLI
ISOLATED FROM
STOOLS OF PATIENTS SUFFERING FROM DIARRHEOA IN
BENIN CITY NIGERIA
Francis Enifo Oronsaye
University of Benin, Nigeria
Background:
Diarrhoea disease is very common in the tropical regions of the world where high temperatures favour bacterial
growth and food are served cold; Moreover, excrement contaminate the environment because low standard of hygiene, E. coli,
abnormal biotype in the gut of humans and animals, but some strains have been incriminated in both bloody and non-bloody
diarrhoea and many of them are toxigenic strains. Thus, the determination of these toxigenic strains cannot be over emphasized.
Methods:
Three hundred stool samples were collected from patients attending various hospitals in Benin City, Nigeria and were
cultured using routine methods of culture and sensitivity in the Medical microbiology department of University of Benin Teaching
Hospital, Benin city, Nigeria.
Results:
The enter virulent isolated were identified to species level using the protocol of Cowan and steel. Antibiotics susceptibility
pattern of the strains were determined using the agar diffusion and dilution methods of Stokes. None of the strain exhibited
high MIC to many antibacterial agents including the fluoroquinolones and were thus subjected to investigation for R-plasmid.
R-plasmid analysis using a horizontal appearance plasmid Tus showed three plasmid bands greater than the reference plasmid
marker.
Conclusion:
This study presents, the isolation of enter virulent
Escherichia coli
that harbour transferable R-plasmid mediated
resistance to the fluoroquinone antibacterial agents and other antibiotics in Benin city, Nigeria.
sayfrankon@yahoo.comJ Gastroenterol Dig Dis 2018, Volume 3