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academies
March 07-09, 2019 | London, UK
2nd International Conference on
7th International Conference on
Food Safety and Hygiene
Nutrition, Food Science and Technology
Joint Event
&
Journal of Food Technology and Preservation | Volume 3
A new high hydrostatic pressure destroyed all pathogens including spores while preserving the
bioactive proteins of donated human milk
Claude Billeaud
Association Européenne de l’Education, France
Background: The main process used to pasteurize human milk
is the low-temperature, long-time Holder method (HOLDER
and recently investigated, the high-temperature, short-time
method). Both processes lead to an appropriated inactivation of
vegetative forms but are ineffective versus the bacterial spores.
Research Aims: Find amethod accomplish twomain objectives:
inactivation of all pathogens, including spores, and preservation
of the activity of milk components.
Design/Methods: Recently, a novel approach of the high
hydrostatic pressure processes have been developed by
HPBioTECH. We compared the effect of human milk treatment
on the same samples (raw human milk, holder and our novel
high hydrostatic pressure) on vegetative and spores forms
of pathogens and on bioactive components (lipase activity,
immunes proteins).
Results: a) Pathogens destructions: two main microbial strains
have been selected: Staphylococcus aureus (as reference for
the vegetative forms) and Bacillus cereus (as reference for
spores). This research led process adapted to the a) microbial
decontamination of 6 log, either for Staphylococcus aureus
or Bacillus cereus, b) Human milk bioactive components: the
main components of human milk is preserved. Activity of the
lipase after this treatment (close to 80%) and that of several
additional components (α-lactalbumin: 96-99%: Casein: 98-
100%, Lysozyme :95-100%; lactoferrin: 93-97%; sIgA: 63-64%).
Conclusions: This novel high hydrostatic process generate
microbiologically safe human milk could potentially result
in important benefits for preterm infants: (i) improved
assimilation of human milk, leading to daily weight and (ii)
improved resistance to infections(iii) to avoid discarding 10% of
contaminated by Bacillus Cereus human milk collected.
Speaker Biography
Claude Billeaud received his MD degree from the Medical University of Bordeaux
(France) in 1979 after a graduation in human cytogenetics (1976). He then studied
pediatrics and has been the Clinical Assistant Director of Bordeaux University in the
departments of Pediatrics, Neonatology and Intensive Care since 1983. He currently
serves as a pediatrician in the neonatal unit at the Children’s Hospital of Bordeaux,
as a scientific manager of Bordeaux-Marmande human milk bank, as a lecturer
and head of research in neonatal nutrition at the Medical University of Bordeaux.
His particular interest in research led him to graduate in Biology and Health (1988,
Bordeaux), be awarded a master in statistics applied to clinical research (1991,
Montreal) and complete a PhD in nutrition and food science (2000, Bordeaux).
Along his career he has often been invited as a guest professor specialized in
nutrition and neonatology in various universities abroad (Montreal, Corrientes
in Argentina). Over the last 35 years, he has been an active member of different
scientific organizations, either French, European or American, specialized in perinatal
medicine (neonatology, pediatrics and nutrition). In this instance, he has served as
the President of the Association for Pediatric Education in Europe (A.P.E.E) since
2008. He has also been very involved in the French human milk banking association
(ADLF) for more than 10 years, sharing his academic knowledge focused in nutrition
and his long clinical experience in neonatology. He is currently carrying out several
researches on the composition of human milk. As an expert in nutrition and perinatal
medicine, he is also the author and co-author of numerous scientific publications.
e:
Claude Billeaud, J Food Technol Pres, Volume 3
DOI: 10.4066/2591-796X-C1-005