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academies
March 07-09, 2019 | London, UK
2nd International Conference on
7th International Conference on
Food Safety and Hygiene
Nutrition, Food Science and Technology
Joint Event
&
Journal of Food Technology and Preservation | Volume 3
Development of species-specific PCR assay for fast fraud detection in seafood: Application to the
authentication of commercially important shrimp species
Lidiya Wilwet
Central Institute of Fisheries Education, India
Shrimps are the important resources from both
commercial fisheries and for aquaculture in many
countries, account for more than 30% of global consumption
of seafood worldwide. The high demand and popularity of
shrimp products have paved way for species substitution in
the commercial market. Identification of species becomes
complicated once its external morphological identification
characteristics are removed particularly in frozen and
precooked shrimp products. Therefore, to enforce labelling
regulations and prevent product substitution, there is a
need for sensitive analytical methods that can be used
to determine the species of a seafood product with no
detectable external features. This study describes a uniplex
PCR assay with species specific primers based on the 16S
rRNA mitochondrial gene to identify the commercially
important shrimp species such as: Fenneropenaes indicus,
Penaeus monodon, P. semisulcatus, Litopenaeus vannamei,
and fresh water prawn Macrobrachium rosenbergii. The
regions which shows maximum inter specific variations were
selected through whole mitochondrial genome analysis and
which paves a way to design five pairs of species-specific
primers based on the 16S rRNA were developed for species
identification. The sensitivity estimation indicated that the
species-specific primers could correctly amplify the target
16S rRNA gene and which yield band sizes of 220, 376, 146,
275 and 750 bp respectively. The specificity of the primers
was very high since it doesn’t cross react with any one of the
closely related species under the same family. The unique
band patterns were also obtained in processed shrimp
products without any degradation or alteration in the major
fragments. The proposedmethodwas also validatedwith 100
shrimp products such as frozen, fried, cooked and canned
shrimp products collected from all over the country. Thus,
the developed protocol can be performed within 2 hrs to
authenticate five shrimp products of commercial significance
so it can be used to expose fraudulent substitution of
processed shrimps in national and international trade.
Speaker Biography
Lidiya Wilwet is doing second year of PhD in Central Institute of Fisheries Education,
Mumbai, India. She is doing research in the field of “Development of Rapid analytical
techniques for finding seafood fraud”. During her M.F. Sc research, she has developed
RFLP markers for commercially important exportable shrimp species viz. Penaeus
monodon, Penaeus semisulcatus, Fenneropenaeus indicus and Litopenaeus vannamei
and which is published in Journal of Food Chemistry through this she acquired Best
Research paper and Technology Development Award from Tamil Nadu Fisheries
University. Apart from this, she published four research papers in reputed journals.
e:
Lidiya Wilwet, J Food Technol Pres, Volume 3
DOI: 10.4066/2591-796X-C1-005