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Journal of Physical Therapy and Sports Medicine
Volume 1, Issue 1
Euro Physiotherapy 2017
Notes:
Page 55
December 07-08, 2017 Rome, Italy
4
th
Euro-Global Physiotherapy Congress 2017
Effect of autologous mesenchymal stem
cell (MSCs) injection on healing of cartilage
defects: Results of preclinical and clinical
studies
Hala Gabr
1
and
Wael Abo El-Kheir
2
1
Cairo University, Egypt
2
Military Medical Academy, Egypt
C
hondrogenesis is a well-orchestrated process derived
by chondroprogenitors that undergo to condensation,
proliferation and chondrocyte differentiation. Because
cartilage lacks blood supply, it lacks regenerative power
and subsequent wound healing. Cartilage degeneration
caused by congenital anomalies, disease or trauma is
of great clinical consequence as it leads to incomplete
attempts of repair by local chondrocytes. The end stage
of cartilage damage frequently leads to O A resulting
in a significant decrease in the quality of life of millions
of people. Treatment methodologies varied from
pharmaceutical, nutraceutical, gene therapy, molecular
engineering; in addition to tissue engineering (TE) also
known as “cell therapy”. In vitro, MSCs showed the
potential to differentiate and can be multiplied without
losing their multilineage capacity of differentiation. This
made the MSCs the cell of choice in tissue engineering.
MSCs are multiline age progenitor cells and responsible
for the turnover and repair of mesenchymal tissues, such
as bone, cartilage, ligament, muscle, and fat. Although no
clear definitive phenotype of MSCs has been described,
through the use of the proper culture conditions, expanded
MSCs can be stimulated to differentiate along specific
pathways, such as chondrogenesis, adipogenesis, and
osteogenesis. The objective of this work was to confirm
the fitness of the dog as a good model of OA; effect of
cell therapy in cases of acute and chronic, compared to
control group surgically induced partial thickness chondral
defects through the injection of autologus bone marrow
derived MSCs in dogs. This work was done on 24 knees
of male domestic mongrel dogs by doing surgical chondral
defects then injected intra-articular with MSCs according
to classified groups: acute (injected after 1 day),chronic
(after 1 month) and control group not injected. The dogs
sacrificed after 1,2,6,8 weeks of injection. Assessment
by histological scoring of cartilage repair (OsScore) for
blind randomized samples and by clinical examination for
lameness degree score. Our results showed that dogs
possess characteristics that are not found in traditional
rodent models and confirmed the efficacy of direct
intraarticular injection of MSCs to home and function in
cartilage defects both in acute and chronic lesions. The
local delivery of MSC is a good therapeutic option for O A.
Biography
Hala Gabr is a renowned Researcher in Stem Cell Biology and Therapy in
Cairo University, Egypt. She is the Director of the Pediatric Bone Marrow
Transplantation and Cellular Therapy Lab in Cairo University. She is the
Co-Founder of the Egyptian Society for Progenitor Stem Cell Research, the
leading stem cell research body in Egypt. She has published more than 30
papers in reputed journals and is an Editorial Board Member of a number of
reputed journals. She has supervised nearly a hundred PhD and master thesis
in stem cell research.
halagabr@yahoo.comHala Gabr et al., J Phys Ther Sports Med 2017