6 / 20
Page 20
allied
academies
J u n e 2 8 - 2 9 , 2 0 1 8 | A m s t e r d a m , N e t h e r l a n d s
Joint Event on
OBESITY AND WEIGHT MANAGEMENT
VACCINES AND IMMUNOLOGY
&
International Conference on
International Conference on
Asian Journal of Biomedical and Pharmaceutical Sciences
|
Volume 8
ISSN:
2249-622X
ENHANCING ANTIBODY
SERODIAGNOSIS ON PEPTIDE
MICROARRAYS USING A CONTROLLED
MULTIPRESENTATION STRATEGY
Marina Cretich
Consiglio Nazionale Delle Ricerche, Italy
H
ere we present a workflow enabling the rapid delivery of efficient
immunoassays for different diagnostics contexts which expands the
current limits of peptide-based serodiagnosis on microarrays. Our strategy
starts from the use of computational tools for accurate immune-reactive
peptide design; exploit chemo-selective strategies for optimal probes
presentation on sensing surfaces using clickable polymeric coatings and
finally generate peptide chips for fluorescence microarrays and SPR imaging.
We will show how the rigorous control of probe design, orientation and
surface density enabled by our platform positively impacts the diagnostic
accuracy of antibody detection in serum of
Burkholderia
infected patients.
Furthermore, we will compare different strategies of peptide multiple
presentation to increase immunoreactivity in the context of allergy screening
and for functional mimicking of discontinuous epitopes of NS1 protein for
Zika virus diagnosis.
Marina Cretich has graduated in Biological Sci-
ence and specialisation in Molecular Biology at
University of Milano in 1998. In 2003, she has
been appointed as Research Scientist at the
Institute of Molecular Recognition Chemistry
of the National Research Council of Italy where
she is currently working as Project Leader of
the protein-based assays division within the
analytical microsystems group. She has been
responsible of national research contracts and
staff scientist in several EC funded projects. Her
scientific activity, documented by more than 70
articles on peer reviewed journals, covers the
field of protein and peptide microarrays for di-
agnostics, protein-protein interaction, biomark-
er discovery/validation; biofunctionalization and
bioconjugation methods for micro-analytical
systems; system integration for biosensing, mi-
crofluidics and point-of-care devices.
marina.cretich@icrm.cnr.itBIOGRAPHY
Marina Cretich, Asian J Biomed Pharmaceut Sci 2018, Volume 8 | DOI: 10.4066/2249-622X-C1-002
Fig.1
Strategy to enhance immunoreactivity by spatially controlled co-presentation
of peptidic probes on microarray surfaces through “click” reaction of yne-
modified peptides on Copoly Azide.