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alliedacademies
J u n e 2 8 - 2 9 , 2 0 1 8 | A m s t e r d a m , N e t h e r l a n d s
Joint Event on
OBESITY AND WEIGHT MANAGEMENT
VACCINES AND IMMUNOLOGY
&
International Conference on
International Conference on
Asian Journal of Biomedical and Pharmaceutical Sciences | Volume 8 ISSN: 2249-622X
IMMUNE RESPONSES OF MICE
IMMUNIZED WITH HBsAg
FORMULATED IN NALOXONE/ALUM
MIXTURE: COMPARISON TO FENDRIX
VACCINE
Mohammad Hossein Yazdi and Hoda Shirazi
Tehran University of Medical Sciences, Iran
Hepatitis B virus can cause cirrhosis of the liver and hepatocellular
carcinoma. Due to the lack of sufficient immune response in whole
population, several researches are being done to improve the efficacy of Alum
based HBV vaccine. Here, naloxone/alum mixture as adjuvant was used for
the HBsAg vaccine and immune parameters evaluated in immunized mice.
In this study the effect of naloxone/alum mixture for the HBsAg vaccine has
been investigated and compared to Fendrix vaccine. Female Balb/cmice were
vaccinated at day 0, 14 and 28 with, alum based vaccine or naloxone/alum
mixture vaccine in different doses. Naloxone/alum vaccine groups received
the dose 3, 6 or 10 mg/kg of naloxone in the vaccine formulation. One group
received routine HBsAg alum vaccine and a group received Fendrix vaccine.
Some groups received naloxone plus HBsAg without alum and a group
received HBsAg without adjuvant. PBS, naloxone and alum were also injected
into the control groups separately. Finally, the naloxone/alum formulated
vaccine compared with the Fendrix and routine alum based vaccine regarding
to the levels of total anti-HBS antibody, IFN-γ, IL-4, IgG1 and IgG2a and the
level of lymphocyte proliferation.The level of total anti-HBS antibody in
Naloxone formulated vaccine was comparable with Fendrix. Meanwhile,
IFN-γ/IL-4 ratio level was significantly higher in Naloxone formulated vaccine
groups versus mere vaccine group. IgG2a was also higher in the naloxone
formulated vaccine groups. These data showed that naloxone/alum mixture
has ability to shift the immune response toward Th1 pattern, which more
potentiate the immunity against infections.
Mohammad Hossein Yazdi has got his PhD
in the field of Pharmaceutical Biotechnology
n 2014 from Tehran University of Medical Sci-
ences, School of Pharmacy. His PhD work was
about cancer treatment by immunotherapy. He
is now Assistant Professor at Biotechnology
Research Center and Recombinant Vaccine Re-
search Center of Tehran University of Medical
Sciences and pursues his interest in both vac-
cine and immunotherapy of cancer and infec-
tious diseases. He has published more than 30
papers in reputed journals and has been serv-
ing as Senior Lecturer of advanced immunolo-
gy and immunotherapy at Tehran University of
Medical Sciences.
BIOGRAPHY
Mohammad Hossein Yazdi et al., Asian J Biomed Pharmaceut Sci 2018, Volume 8 | DOI: 10.4066/2249-622X-C1-002