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Note:
Ethnopharmacology 2019
& Physiotherapy Congress 2019
Asian Journal of Biomedical and Pharmaceutical Sciences
ISSN: 2249-622X | Volume 9
Page 41
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
March 27-28, 2019 | Amsterdam, Netherlands
&
PHARMACOLOGY AND ETHNOPHARMACOLOGY
7th International Conference and Exhibition on
5th GLOBAL PHYSIOTHERAPY, PHYSICAL
REHABILITATION AND SPORTS MEDICINE
Joint Event on
ANALYSIS OF MYOSIN-ATPASE ACTIVITY AND SATELLITE’S CELL ACTIVATION
IN SOLEUS MUSCLE AFTER SURGICAL DENERVATION OFWISTAR RAT
Sabrina Degaspari1 and Renato Paulo Chopard2
1Centro Universitario Lusiada, Brazil
2Sao Paulo University, Brazil
S keletal muscle denervation compounds a group of affection of locomotor system capable to promote im-
portant physical disabilities. We studied the effects of muscle denervation on different fiber types, mor-
phology of satellite cell and microvascular supply in soleus muscle of Wistar rats after surgical neural dam-
age through sciatic nerve section. After fifteen days of hindlimb palsy, the soleus muscle atrophy was studied
through the analisys of muscular weight and transverse sectional area of muscle fibers. Following, we analyzed
the diferent fiber types using histochemical analyses for myosin-ATPase reaction to appoint changes in con-
tractile behavior of soleus muscle. The mophology of satellite’s cells was studied using electron transmission
microscopy images and the microvasculature of the tissue was analyzed obtained histological reactions for
toluidine blue to study the rate of capilar-fiber. We observed that the soleus muscle showed reduction of 36%
of mass as well as 50% of reduction in the cross sectional area in the experimental group after fifteen days of
sciatic nerve damage, indicating muscle hypotrophy. Also, we verified an increase in the fast-twitch (type IIb)
and undifferentiated fiber types revealing alteration in the muscle contractile behavior. The electron transmis-
sion microscopy revealed disarrangement of the skeletal muscle tissue with the one sided prolongations of
satellite cell, showing its migratory potential and capacity to regenerating the lesion’s tissue. We compared all
this results with the capillary-fiber rates finding 35% less capillary per muscle fiber in control animals that in the
experimental animals. We conclude that keep up with the change in the muscle contraction pattern this study
showed a microvascularization adjustment consistent with the histochemical modification of soleus muscle
during atrophy process.
Sabrina Degaspari et al., Asian J Biomed Pharmaceut Sci 2019, Volume 9 | DOI: 10.4066/2249-622X-C1-017
Sabrina Degaspari is formed in physical therapy and pharmacology, has completed her master degree in morphology,
concentration area anatomy at Sao Paulo University and PhD in biosciences, concentration area neuropharmacolo-
gy at Sao Paulo University. She teaches at Centro Universitario Lusiada and Santa Cecilia University and attends as a
phisyoterapist at Corpo e Agua clinic.
BIOGRAPHY