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Page 30
allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
Deciphering spiral ganglion neurons heterogeneity by single-neuron transcriptome profiling
Mirna Mustapha
Stanford University, USA
T
ype I spiral ganglion neurons (SGNIs) are myelinated
and carry all the auditory information from organ
of Corti inner hair cells to the auditory brainstem. SGNIs
are morphologically and physiologically diverse; they
consist of low and high threshold subtypes. Moreover,
they differ in their susceptibility to noise insult. These
differences between types and subtypes are likely
driven by distinct gene expression profiles. We used a
transgenic mouse with the unique feature of fluorescently
labeled SGNI and single cell gene expression profiling
to distinguish different SGNI subtypes. We employed
quantitative single cell RT-PCR for a preselected group
of 192 candidate genes aimed to specifically distinguish
among subtypes of SGNIs. These studies will contribute
to elucidate significant questions in the field such as
molecular profiles that define the different SGNI subtypes.
e
:
mirnam@stanford.eduENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003