ent-stem-cell-2018-posters-abstracts

Page 30

allied

academies

September 10-11, 2018 | Paris, France

Joint Event

Otolaryngology: ENT Surgery

International Conference on

Cell & Stem Cell Research

World Congress and Expo on

Journal of Otolaryngology Online Journal | Volume 8

Deciphering spiral ganglion neurons heterogeneity by single-neuron transcriptome profiling

Mirna Mustapha

Stanford University, USA

ype I spiral ganglion neurons (SGNIs) are myelinated

and carry all the auditory information from organ

of Corti inner hair cells to the auditory brainstem. SGNIs

are morphologically and physiologically diverse; they

consist of low and high threshold subtypes. Moreover,

they differ in their susceptibility to noise insult. These

differences between types and subtypes are likely

driven by distinct gene expression profiles. We used a

transgenic mouse with the unique feature of fluorescently

labeled SGNI and single cell gene expression profiling

to distinguish different SGNI subtypes. We employed

quantitative single cell RT-PCR for a preselected group

of 192 candidate genes aimed to specifically distinguish

among subtypes of SGNIs. These studies will contribute

to elucidate significant questions in the field such as

molecular profiles that define the different SGNI subtypes.

ENT and Stem Cell 2018, Volume 8

DOI: 10.4066/2250-0359-C1-003