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allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
Functional improvement of hemostatic scaffold by addition of recombinant batroxobin
Gyeung Mi Seon, Mi Hee Lee, Min-Ah Koo, Seung Hee Hong
and
Jong-Chul Park
Yonsei University College of Medicine, Republic of Korea
U
ncontrolled hemorrhaging is the leading preventable
cause of infectious complications in accidents, surgical
procedures and battle fields. It indicates that excessive
blood bleeding could ultimately be correlated with life.
Therefore, topical hemostats and sealants are required
that they can assist the effective treating arrest bleeding
and stabilize the casualty. In many types of hemostatic
agents, collagen and chitosan play an important role for clot
formation and platelet activation and aggregation in blood
coagulation. Additionally, chitosan adheres to red blood
cell in blood coagulation. However, some studies have been
reported natural substances were not adequately quick to
hemostasis within relevant time. Thus, this study focused
on recombinant batroxobin (rBat), from cDNA expressed
in Pichia pastoris. In the hemostat dressing, rBat functions
as fibrinogen to convert enzymes, plasminogen activators,
prothrombin activators, factor X activators, or hemorrhagins
such as thrombin. rBat interacts with proteins in the blood
coagulation cascade and acts specifically on the fibrinolytic
pathway. In contrast to thrombin, batroxobin splits off
only the fibrinopeptide α-chain, leaving the β-chain of
fibrinogen unaffected. In addition, the rBat, thrombin-like
enzyme components, does not influence other hemostatic
factors or cells. Therefore, in this study, we fabricated a
novel collagen and chitosan hemostatic scaffold containing
rBat using freeze-dry method. Because each of the
materials used in the scaffold involve different hemostatic
mechanisms within the coagulation cascade, the scaffold
was predicted to be effective at controlling bleeding. Also,
incorporation of rBat was predicted to provide a synergetic
effect to natural substances for induce rapid hemostasis.
Experiments performed here in vitro studies to evaluate
respective hemostatic mechanism confirm the efficacy of
this novel hemostatic scaffold. For animal experiments,
we used a Sprague-Dawley (SD) rat initial hemorrhage
model. In conclusion, the scaffold dressing should be
a definite improve control of excessive hemorrhage.
Speaker Biography
Gyeung Mi Seon is currently pursuing in the Yonsei University College of Medicine at
Republic of Korea.
e:
Parkjc@yuhs.acENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003