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allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
Homogeneous distribution of hMSC in 3D PCL scaffold by electrical stimulation
Mi Hee Lee, Min Sung Kim, Byeong-Ju Kwon, Min-Ah Koo, GyeungMi Seon, Dohyun Kim, Seung Hee Hong, Jeong Koo Kim
and
Jong-Chul Park
Yonsei University College of Medicine, Republic of Korea
T
issueengineeringhasshowntremendouspromiseincreating
biological alternatives for harvested tissues, implants, and
prostheses. An ideal scaffold for tissue regeneration should
possess large porosity and pore size for good infiltration of cells,
high pore interconnectivity for tissue ingrowth. Cell culture
in 3-D porous scaffolds is often impaired by the difficulty of
achieving a homogeneous cell seeding and by the diffusion
constraints within the cell-scaffold constructs. In the present
study, we investigated the effect of electric stimulation on
the migration of hMSCs and infiltration of hMSCs into 3D PCL
scaffold by electric stimulation. To fabricate the porous scaffold,
we simulated the current distribution in designed scaffold
using comsol physics computer program. We designed lattice
structure for difficult infiltration without any stimulation.
Then, 3D PCL scaffolds was fabricated by 3D printing. During
electrotaxis on 2D, hMSCs moved toward the anode or cathode
under direct current electric fields. Cell seeded into PCL
scaffold, incubated for 1day, and then treated 1000 μA electric
for 3h using a customized agar-salt electrotaxis chamber. After
electric current treatments, cell distribution on PCL scaffold
were visualized by immunofluorescence staining. As a result, if
there was no electric treatment, cell stayed near the surface of
scaffold. However, electric stimulation enhanced the infiltration
of cells into scaffold and hMSC distributed and proliferated
on 3D scaffolds for 28d, homogenously. In conclusion, the
infiltration of hMSCs into scaffold was enhanced by the control
ofmigrationusing physical stimulations as electrical stimulation.
Speaker Biography
Mi Hee Lee is currently pursuing in the Yonsei University College of Medicine at Republic
of Korea.
e:
parkjc@yuhs.acENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003